Overview

  • Product name

  • Description

    Rabbit polyclonal to NOA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human NOA1 aa 400-450. The exact sequence is proprietary.
    Database link: Q8NC60

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab226402 has not performed satisfactorily when used for WB of NOA1 in crude preparations (e.g. whole cell lysate).  This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NOA1.

     This product was previously labelled as C4orf14

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      The exact function of C4orf14 is currently unknown
    • Database links

    • Alternative names

      • Chromosome 4 open reading frame 14 antibody
      • hAtNOS1 antibody
      • hNOA1 antibody
      • Hypothetical protein LOC84273 antibody
      • mAtNOS1 antibody
      • MGC3232 antibody
      • MTG3 antibody
      • Nitric oxide associated 1 antibody
      • NOA1 antibody
      • Putative ortholog of Arabidopsis mitochondrial nitric oxide synthase antibody
      • Uncharacterized protein C4orf14 antibody
      see all

    Images

    • NOA1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226402 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226402 at 1 µg/ml.

      Lane 1: ab226402 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 minutes.

    References

    ab226402 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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