Key features and details
- Rabbit polyclonal to NOA1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NOA1 antibody
See all NOA1 primary antibodies
DescriptionRabbit polyclonal to NOA1
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey
Synthetic peptide within Human NOA1 aa 400-450. The exact sequence is proprietary.
Database link: Q8NC60
- IP: HEK-293T whole cell lysate.
ab226402 has not performed satisfactorily when used for WB of NOA1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NOA1.
This product was previously labelled as C4orf14
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226402 was affinity purified using an epitope specific to NOA1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceThe exact function of C4orf14 is currently unknown
- Chromosome 4 open reading frame 14 antibody
- hAtNOS1 antibody
- hNOA1 antibody
NOA1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226402 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226402 at 1 µg/ml.
Lane 1: ab226402 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226402 has not yet been referenced specifically in any publications.