Key features and details
- Rabbit polyclonal to NOB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NOB1 antibody
See all NOB1 primary antibodies
DescriptionRabbit polyclonal to NOB1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey, Orangutan
- WB: HeLa whole cell lysate; Mouse brain, kidney and stomach tissue lysates. IHC-P: Human thyroid and placenta tissues. ICC/IF: A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab224619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionMay play a role in mRNA degradation.
Tissue specificityDetected in liver, lung, placenta, endothelial cells and spleen.
Sequence similaritiesBelongs to the NOB1 family.
Contains 1 PINc domain.
- Information by UniProt
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All lanes : Anti-NOB1 antibody (ab224619) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse stomach tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Paraffin-embedded human thyroid tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for NOB1 using ab224619 at 1/100 dilution in immunohistochemical analysis.
A549 (human lung carcinoma cell line) cells stained for NOB1 (green) using ab224619 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224619 has been referenced in 3 publications.
- Liu H et al. MicroRNA-744 suppresses cell proliferation and invasion of papillary thyroid cancer by directly targeting NOB1. Mol Med Rep 19:1903-1910 (2019). PubMed: 30628685
- Zhang J et al. LncRNA OIP5-AS1 Promotes the Proliferation of Hemangioma Vascular Endothelial Cells via Regulating miR-195-5p/NOB1 Axis. Front Pharmacol 10:449 (2019). PubMed: 31068824
- Deng Y et al. microRNA-744 is downregulated in glioblastoma and inhibits the aggressive behaviors by directly targeting NOB1. Am J Cancer Res 8:2238-2253 (2018). PubMed: 30555741