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    noc4l-antibody-pcrp-noc4l-1e3-ab277096.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Other
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Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)

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Immunocytochemistry - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)
  • Flow Cytometry - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)
  • Protein Array - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)

Key features and details

  • Mouse monoclonal [PCRP-NOC4L-1E3] to NOC4L
  • Suitable for: Protein Array, Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-NOC4L antibody [PCRP-NOC4L-1E3]
    See all NOC4L primary antibodies
  • Description

    Mouse monoclonal [PCRP-NOC4L-1E3] to NOC4L
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, Flow Cyt, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human NOC4L aa 1-516.
    Database link: Q9BVI4

  • Positive control

    • Flow cyt: HeLa cells. ICC: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99.9% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate
  • Clonality

    Monoclonal
  • Clone number

    PCRP-NOC4L-1E3
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Alternative Versions

    • Anti-NOC4L antibody [PCRP-NOC4L-1E3] - BSA and Azide free (ab277112)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Our Abpromise guarantee covers the use of ab277096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells.
ICC Use a concentration of 1 - 2 µg/ml.

Target

  • Sequence similarities

    Belongs to the CBF/MAK21 family.
  • Cellular localization

    Nucleus membrane. Nucleus > nucleolus.
  • Target information above from: UniProt accession Q9BVI4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 79050 Human
    • Omim: 612819 Human
    • SwissProt: Q9BVI4 Human
    • Unigene: 558536 Human
    • Alternative names

      • AI326906 antibody
      • MGC28606 antibody
      • MGC3162 antibody
      • NET49 antibody
      • NOC4 antibody
      • NOC4 like protein antibody
      • NOC4 protein homolog antibody
      • NOC4-like protein antibody
      • Noc4l antibody
      • NOC4L_HUMAN antibody
      • Nucleolar complex associated 4 homolog (S. cerevisiae) antibody
      • Nucleolar complex protein 4 homolog antibody
      • Nucleolar complex-associated protein 4 like protein antibody
      • Nucleolar complex-associated protein 4-like protein antibody
      • OTTMUSP00000032269 antibody
      • RGD1310661 antibody
      see all

    Images

    • Immunocytochemistry - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)
      Immunocytochemistry - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)

      Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling NOC4L with ab277096 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

    • Flow Cytometry - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)
      Flow Cytometry - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)

      Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling NOC4L using ab277096 at 2 µg / 106  cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

    • Protein Array - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)
      Protein Array - Anti-NOC4L antibody [PCRP-NOC4L-1E3] (ab277096)

      Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277096.

      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab277096? Please let us know so that we can cite the reference in this datasheet.

    ab277096 has not yet been referenced specifically in any publications.

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