The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia. Orphanin FQ2: Has potent analgesic activity.
Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Belongs to the opioid neuropeptide precursor family.
Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Dual labeling of Nociceptin (green) and enkephalin (red). 7 μm paraffin sections of human brain tissue were cut and floated onto positively charged slides. After being dewaxed and microwaved in 10mM citric acid (pH 6.0) for 20 minutes at 600W, sections were incubated with the rabbit polyclonal antisera overnight at 4°C, primary antibody was detected using biotinylated goat anti-rabbit IgG followed by incubation with peroxidase-conjugated avidin.
Binding of the primary antibody was visualized using 3-amino-9-ethylcarbazole (AEC) in acetate buffer. Afterwards, sections were rinsed, counterstained with Mayer`s hematoxylin and mounted in Vectamount mounting medium.