Key features and details
- Rabbit polyclonal to Nociceptin
- Suitable for: IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Nociceptin antibody
See all Nociceptin primary antibodies
DescriptionRabbit polyclonal to Nociceptin
Tested applicationsSuitable for: IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Dog, Pig
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab100880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionNociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.
Orphanin FQ2: Has potent analgesic activity.
Tissue specificityPredominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Sequence similaritiesBelongs to the opioid neuropeptide precursor family.
modificationsSpecific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
- Information by UniProt
- N/OFQ antibody
- N23K antibody
- N23K/N27K antibody
Dual labeling of Nociceptin (green) and enkephalin (red). 7 μm paraffin sections of human brain tissue were cut and floated onto positively charged slides. After being dewaxed and microwaved in 10mM citric acid (pH 6.0) for 20 minutes at 600W, sections were incubated with the rabbit polyclonal antisera overnight at 4°C, primary antibody was detected using biotinylated goat anti-rabbit IgG followed by incubation with peroxidase-conjugated avidin.
Binding of the primary antibody was visualized using 3-amino-9-ethylcarbazole (AEC) in acetate buffer. Afterwards, sections were rinsed, counterstained with Mayer`s hematoxylin and mounted in Vectamount mounting medium.
ab100880 has not yet been referenced specifically in any publications.