Anti-Nociceptin antibody (ab6174)

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Overview

  • Product name
    Anti-Nociceptin antibody
    See all Nociceptin primary antibodies
  • Description
    Rabbit polyclonal to Nociceptin
  • Host species
    Rabbit
  • Specificity
    Achieved by producing antibody only against the C terminal portion of the full peptide that avoids possible cross-reaction with enkephalin, dynorphin and endorphin. Specificity was tested by the use of various peptides for adsorption in immunohistochemistry including enkephalin, dynorphin and endorphin.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, RIAmore details
  • Species reactivity
    Reacts with: Rat, Guinea pig
  • Immunogen

    Synthetic peptide CTGARKSARKLANQ corresponds to rat nociceptin protein with a cysteine added at the N terminal for conjugation to diphtheria toxoid. The peptide was cross-linked by maleimidocaproyl-N-hydroxy succinimide.

  • General notes
    This antibody can be used for staining nerve fibres in guinea pig myenteric plexus. Staining was established with the use of fluorescence immunohistochemistry (CY3).

Properties

Applications

Our Abpromise guarantee covers the use of ab6174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
RIA 1/2500. PubMed: 17478889

Target

  • Function
    Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
    Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.
    Orphanin FQ2: Has potent analgesic activity.
  • Tissue specificity
    Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
  • Sequence similarities
    Belongs to the opioid neuropeptide precursor family.
  • Post-translational
    modifications
    Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • N/OFQ antibody
      • N23K antibody
      • N23K/N27K antibody
      • Nociceptin, included antibody
      • nocistatin antibody
      • Npnc1 antibody
      • OFQ antibody
      • OFQ/N antibody
      • Orphanin FQ antibody
      • Orphanin FQ2 antibody
      • Pnoc antibody
      • PNOC_HUMAN antibody
      • ppN/OFQ antibody
      • PPNOC antibody
      • pre-pro-N/OFQ antibody
      • prepronociceptin antibody
      • pronociceptin antibody
      • Propronociceptin antibody
      see all

    References

    This product has been referenced in:
    • Dai L  et al. SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia. Mol Cell Biol 37:N/A (2017). Read more (PubMed: 28031330) »
    • Sanathara NM  et al. Estradiol upregulates progesterone receptor and orphanin FQ colocalization in arcuate nucleus neurons and opioid receptor-like receptor-1 expression in proopiomelanocortin neurons that project to the medial preoptic nucleus in the female rat. Neuroendocrinology 100:103-18 (2014). Read more (PubMed: 24821192) »
    See all 3 Publications for this product

    Publishing research using ab6174? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Question

    I recently placed an order for AB6174 and have received it (impressively fast) last week. Anyhow, the information provided on the site mentions possible cross-reaction with enkephalin, dynorphin, and endorphin. We would like to do some testing in regards to this and was wondering if you can give me the catalog numbers for those 3 peptides or recommendations to where to obtain the

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    Answer

    Thank you for contacting Abcam. Again, I am sorry for the delay in getting this information to you. At this time we do not offer the peptides for endorphin, dynorphin and enkephalin used in the testing of this product. I would like to suggest that you try biocompare.com to search for these peptides. If you have any questions please feel free to contact me.

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    Question

    Hello, Was wondering if someone could please provide me with the request below? Or call me. Thanks,

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    Answer

    Thank you for contacting Abcam. I did leave a message on your voicemail but wanted to also send you a written response. I am sorry that I have been unable to find the information that you have requested as yet. I have contacted the lab about obtaining the peptides used to test for cross reactivity or if this is a predicted but not tested result. I will let know know the moment I hear anything back from them. Again, I apologise for the wait. Please let me know if you have any questions or if there is anything else that I may do for you.

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    Question

    Can you please send me details of the differances between this product and ab10276. Do they both select for the same nociceptin?

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    Answer

    Thank you for your enquiry. Below are the main differences between these two antibodies. Ab10276 - Guinea pig polyclonal to Nociceptin - was generated using the following inmmunogen: Synthetic peptide: FGGFTGARKSARKLANQ, corresponding to amino acids 122-138 of Rat Orphanin FQ. The antibody reacts with rat and primate and has been tested for application in Immunohistochemistry (Frozen sections). Ab6174 - Rabbit polyclonal to Nociceptin - was generated using the following immunogen: Synthetic peptide CTGARKSARKLANQ corresponds to rat nociceptin protein with a cysteine added at the N terminal for conjugation to diphtheria toxoid. The peptide was cross-linked by maleimidocaproyl-N-hydroxy succinimide. The antibody reacts with guinea pig and rat and has been tested for application in ELISA, Western blot and Immunohistochemistry (Frozen sections). I hope this helps. Please contact us again if you have any additional questions.

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    Question

    Would you kindly tell me what is 17aa?

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    Answer

    Thank you for your enquiry. "17aa" refers to 17 amino acids. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    At what antibody concentration is the delivered 100ul of liquid?

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    Answer

    We use an estimate for anti-sera since there is no routine technique available for measuring the fraction of specific IgG to your target. The amount of IgG in whole anti-sera is around 10mg/ml and the amount of specific IgG is normally around 5-10% so we estimate a concentraion of specific antibody at 0.5-1.0 mg/ml.

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    Question

    Just wondering what you calculated the titre for this antibody to be? and how you'd define titre? Many thanks, Emma, PhD student, Leicester

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    Answer

    It depends on the application in which you intend to use the antibody. For IHC and 1-site ELISA, a working dilution of 1/3000 is suggested (based on work carried out by the originator of this antibody), however it is always a good idea to optimise in your own laboratory using this dilution as a starting point.

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    Question

    I am wondering about the ELISA method used to test this antibody - is it the same as that under the protocols section? If so, when using 6174 as the primary antibody, what dilution would you recommend? Many thanks, Emma PhD Student, Leicester

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    Answer

    This antibody has been tested in a 1-site ELISA only; the recommended dilution is 1/3000. The protocols in the protocols section are general guidelines.

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