Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab66219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/400.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-Fr 1/40 - 1/100.

Target

  • Function

    Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • H.sapiens mRNA for ORL1 receptor antibody
    • Kappa type 3 opioid receptor antibody
    • Kappa-type 3 opioid receptor antibody
    • kappa3-related opioid receptor antibody
    • KOR 3 antibody
    • KOR-3 antibody
    • KOR3 antibody
    • LC132 receptor like antibody
    • LC132 receptor, rat, homolog of antibody
    • MGC34578 antibody
    • Nociceptin receptor antibody
    • Nociceptin/orphanin FQ (N/OFQ) peptide receptor antibody
    • NOCIR antibody
    • NOP antibody
    • OOR antibody
    • Opiate receptor like 1 antibody
    • Opiod receptor like 1 antibody
    • Oprl1 antibody
    • OPRX_HUMAN antibody
    • ORL1 antibody
    • Orphanin FQ receptor antibody
    see all

Images

  • Sk-N-SH cells were incubated at 37°C for 24h with vehicle control (0 µM) and 1 µM of kainic acid (ab120100). Decrease expression of nociceptin receptor in SK-N-SH cells correlates with an increase in kainic acid concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab66219 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

References

This product has been referenced in:

  • Wang K  et al. Nociceptin Receptor Is Overexpressed in Non-small Cell Lung Cancer and Predicts Poor Prognosis. Front Oncol 9:235 (2019). Read more (PubMed: 31024840) »
  • Wheway G  et al. An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nat Cell Biol 17:1074-87 (2015). ICC/IF . Read more (PubMed: 26167768) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Human Cell lysate - whole cell (retinal pigment epithelium, fibroblasts, neuronal,)
Specification
retinal pigment epithelium, fibroblasts, neuronal,
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 04 2013

Answer

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

Isotype controls:



can be searched at this site: https://www.abcam.com/index.html?c=718

ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032



Positive control:



ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

ab66219 -- Oprl1 - Brain (cortex),

ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your enquiry and your interest in our products.

This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have contacted the lab to get the requested information for you concerning ab66219 (Lot: GR9812).

Would you be so kind to clarify if you wish to look for isotype control or positive control for these primary antibodies listed below?

ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG

I look forward to hearing from you soon. As soon as I have further information on the concentration (ab66219), I will contact you again.

Read More

Answer

Thank you again for contacting us, and I apologize for the extended delay regarding these peptides.

The blocking peptide for ab66219 is now available in our catalog as ab129205-

https://www.abcam.com/Oprl1-peptide-ab129205.html

I am still working on having the blocking peptides for ab77384, ab77384, and ab65700 added to the catalog, and I hopeto have this done shortly.

As I mentioned before, ab66219 has not been tested in IHC-P but it may be suitable for this application. If you are interested in trying this antibody in IHC-P, we do have a testing program available. In this program, you would receive a free primary antibody on a future purchase after purchasing and testing ab66219 then submitting an Abreview with your data. If you are interested in this program, I would be happy to send more information.

I will get back in touch with you once I have more information about the peptides for the other antibodies. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Answer

Thank you for contacting us last week and for your patience while I have been working onadding these blocking peptides to our catalog. I apologize for the extended delay getting back to you.

Thesepeptides will be added to our catalog shortly, and I will then email you thecatalog numbers so you will be able to order them. Regarding ab66219, we have not tested it in IHC-P but it iscertainly possible that it will work. The antigen retrieval method and dilution factor may need to be optimized, but if you have any trouble please let me know and I'll be happy to help you optimize the conditions.

I will be in touch soon. Please let me know if you haveany questions in the meantime, and I'll be happy to help.

Read More

Answer

My colleague Teresa has forwarded your request to our team.

I have checked the datasheets for the PFA- perfusion fixed frozen section application (IHC-FoFr), andthe antibodiesare all not tested for this. This means unfortunately, that we cannot guarantee the outcome.

However,I can offer a discount off a future purchase if you buyab36204, ab66219, ab83508and /or ab102998now, test it in IHC-FoFr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 freeprimary antibodyper tested antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab36204, ab66219, ab83508 and /or ab102998 in IHC-FoFr. I will then send a discount code. This code must be issued before purchasing ab36204, ab66219, ab83508 and /or ab102998 so please wait for my reply before ordering.

2. Purchase ab36204, ab66219, ab83508 and /or ab102998 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-FoFr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab36204, ab66219, ab83508 and /or ab102998 turns out to be unsuitable for IHC-FoFr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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