Overview

  • Product name
    Nocodazole, Microtubule inhibitor
  • Description
    Microtubule inhibitor
  • Biological description
    Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.
  • Purity
    > 98%
  • CAS Number
    31430-18-9
  • Chemical structure
    Chemical Structure

Properties

  • Chemical name
    Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate
  • Molecular weight
    301.32
  • Molecular formula
    C14H11N3O3S
  • PubChem identifier
    4122
  • Storage instructions
    Store at +4°C. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in DMSO to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
  • Source

    Synthetic

Images

  • All lanes : Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 23 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
    Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab120630 has not yet been referenced specifically in any publications.

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