• Product name

  • Description

    Rabbit polyclonal to Nocturnin
  • Host species

  • Specificity

    Although there is a high homology with mouse, this antibody also has the potential to cross react with another mouse protein of similar molecular weight therefore this antibody is not suitable for use in mouse.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    A region within a synthetic peptide: RLYSALAKTL NSSAASQHPE YLVSPDPEHL EPIDPKELLE ECRAVLHTRP, corresponding to N terminal amino acids 73-122 of Human Nocturnin

  • Positive control

    • Human lung tissue (IHC-P), Daudi cell lysate (western blot).


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab22850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • Relevance

    CCRN4L is highly similar to Nocturnin, a gene identified as a circadian clock regulated gene in Xenopus laevis. This protein and Nocturnin protein share similarity with the C terminal domain of a yeast transcription factor, carbon catabolite repression 4 (CCR4). The mRNA abundance of a similar gene in mouse has been shown to exhibit circadian rhythmicity, which suggests a role for this protein in clock function or as a circadian clock effector.
  • Database links

  • Alternative names

    • CCR4 carbon catabolite repression 4 like (S. cerevisiae) antibody
    • CCR4 protein homolog antibody
    • CCRN4L antibody
    • NOC antibody


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Nocturnin with ab22850 at 4.0-8.0µg/ml. Positive staining seen in the bronchiole epithelium. Magnification: 400X.
  • Anti-Nocturnin antibody (ab22850) at 4 µg/ml + Daudi cell lysate

    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

    Gel concentration: 12%
  • Paraffin-embedded Human lung tissue. Cells with positive label: epithelial cells of bronchiole (indicated by arrows). Antibody concentration: 4-8µg/ml. Magnification: 400x.


ab22850 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Mouse Cell lysate - whole cell (3T3 cells (fibroblasts))
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
3T3 cells (fibroblasts)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2015


Thank you for getting back to me with further information. I have read your previous questionnaire and the further details that you have provided me with. I find your enquiry most interesting. As I can see you are ONLY detecting the correctly sized and expressed Nocturin following immunoprecipitation. The western blots that you have performed are clearly detecting extraneous bands but not the FLAG tagged product when using this antibody. However, the Flag antibody detects the correctly sized Nocturin and demonstrates an enrichment following IP. The recognition of the FLAG tagged expressed Nocturin by western blot analysis is obviously the root of the problem. I ask under what conditions have you performed the detection. I ask this because we often find diluting the antibody in BSA with an overnight incubation at 4oC leads to heightened specificity by western blotting. I look forward to hearing from you.

Read More


Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. You mention that this antibody has been detecting bands other than overexpressed murine and human nocturin proteins. Please can you tell me whether these bands could possibly be degradation products as I would recommend that the lysis buffer is supplemented with additional protease inhibitors. I would also appreciate your comments on whether these extraneous bands could be the result of truncated products from your expression construct. Please can you tell me whether you have eliminated this possibility by probing with an antibody against the FLAG tag. (This assumes that the FLAG is positioned at the N terminus; else FLAG will ONLY detect the full sized protein). In your questionnaire you mentioned that you thought that this antibody was detecting bands other than Nocturnin. Can you tell me whether there are bands detected in the negative controls? Finally I would greatly appreciate it if you could e-mail me an image of a representative blot as this will greatly assist me in understanding the pattern of extraneous bands that you have been detecting. I look forward to hearing from you.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up