Overview

  • Product name

    Anti-NOD1 antibody [EPR20833]
    See all NOD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20833] to NOD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse NOD1 aa 300-400. The exact sequence is proprietary.
    Database link: Q8BHB0

  • Positive control

    • WB: Wild-type BMDM whole cell lysate; mouse lung tissue lysate.
  • General notes

     This product was previously labelled as CARD4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20833
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 107 kDa).

Target

  • Function

    Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection.
  • Tissue specificity

    Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Post-translational
    modifications

    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition.
  • Cellular localization

    Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • CARD 4 antibody
    • CARD4 antibody
    • Caspase recruitment domain 4 antibody
    • Caspase recruitment domain containing protein 4 antibody
    • Caspase recruitment domain family member 4 antibody
    • Caspase recruitment domain protein 4 antibody
    • Caspase recruitment domain-containing protein 4 antibody
    • CLR 7.1 antibody
    • CLR7.1 antibody
    • NLR family CARD domain containing 1 antibody
    • NLRC 1 antibody
    • NLRC1 antibody
    • NOD 1 antibody
    • Nod1 antibody
    • NOD1 protein antibody
    • NOD1_HUMAN antibody
    • Nucleotide binding oligomerization domain containing 1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody
    • Nucleotide-binding oligomerization domain-containing protein 1 antibody
    • Protein Nod1 antibody
    see all

Images

  • Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution + Mouse lung tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    NOD1 is an alternative name for CARD4.

    The 100-kDa band is the specific NOD1 band, other bands are unspecific.

    The lysates were un-boiled to avoid the protein aggregation.

  • All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution

    Lane 1 : Wild-type BMDM (mouse bone marrow-derived macrophages), whole cell lysate
    Lane 2 : NOD1 knockout BMDMs, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 107 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    The 100-kDa band is the specific NOD1 band, other bands are unspecific.

    NOD1 is an alternative name for CARD4.

    The WT and NOD1 KO BMDMs lysates were kindly provided by our collaborator Dr. Gang Liu. Tsinghua University.

References

ab215726 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215726.
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