Anti-Nodal antibody (ab227052)
Key features and details
- Rabbit polyclonal to Nodal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nodal antibody
See all Nodal primary antibodies -
Description
Rabbit polyclonal to Nodal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human Nodal (internal sequence). The exact sequence is proprietary.
Database link: Q96S42 -
Positive control
- IHC-P: Human breast cancer tissue; rat brain tissue. WB: Human ESC whole cell lysate; mouse brain tissue extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
Target
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Function
Essential for mesoderm formation and axial patterning during embryonic development. -
Involvement in disease
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4838 Human
- Entrez Gene: 18119 Mouse
- Entrez Gene: 294503 Rat
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- SwissProt: P43021 Mouse
- Unigene: 370414 Human
- Unigene: 57195 Mouse
see all -
Alternative names
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
Images
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Western blot - Anti-Nodal antibody (ab227052)Anti-Nodal antibody (ab227052) at 1/1000 dilution + Human embryonic stem cell (ESC) whole cell lysate at 1/20 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nodal antibody (ab227052)Paraffin embedded human breast cancer tissue stained for Nodal with ab227052 (1/250 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nodal antibody (ab227052)Paraffin embedded rat brain tissue stained for Nodal with ab227052 (1/500 dilution) in immunohistochemical analysis.
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Western blot - Anti-Nodal antibody (ab227052)Anti-Nodal antibody (ab227052) at 1/500 dilution + Mouse brain tissue extracts at 50 µg
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab227052 has not yet been referenced specifically in any publications.
