Anti-Nodal antibody (ab227052)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Nodal antibody
See all Nodal primary antibodies -
Description
Rabbit polyclonal to Nodal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human Nodal (internal sequence). The exact sequence is proprietary.
Database link: Q96S42 -
Positive control
- IHC-P: Human breast cancer tissue; rat brain tissue. WB: Human ESC whole cell lysate; mouse brain tissue extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
Target
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Function
Essential for mesoderm formation and axial patterning during embryonic development. -
Involvement in disease
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4838 Human
- Entrez Gene: 18119 Mouse
- Entrez Gene: 294503 Rat
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- SwissProt: P43021 Mouse
- Unigene: 370414 Human
- Unigene: 57195 Mouse
see all -
Alternative names
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
Images
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Western blot - Anti-Nodal antibody (ab227052)Anti-Nodal antibody (ab227052) at 1/1000 dilution + Human embryonic stem cell (ESC) whole cell lysate at 1/20 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nodal antibody (ab227052)Paraffin embedded human breast cancer tissue stained for Nodal with ab227052 (1/250 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nodal antibody (ab227052)Paraffin embedded rat brain tissue stained for Nodal with ab227052 (1/500 dilution) in immunohistochemical analysis.
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Western blot - Anti-Nodal antibody (ab227052)Anti-Nodal antibody (ab227052) at 1/500 dilution + Mouse brain tissue extracts at 50 µg
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS-PAGE
Datasheets and documents
References
ab227052 has not yet been referenced specifically in any publications.
