Product nameAnti-Nodal antibody [EP2058Y]
See all Nodal primary antibodies
DescriptionRabbit monoclonal [EP2058Y] to Nodal
Specificityab81287 detects Pro-Nodal and Nodal.
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Nodal aa 300-400 (C terminal). The exact sequence is proprietary.
- Recombinant nodal protein.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab81287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/2000. Predicted molecular weight: 39 kDa. (Pro-Nodal) and 13 kDa (Nodal).|
FunctionEssential for mesoderm formation and axial patterning during embryonic development.
Involvement in diseaseDefects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution + Recombinant nodal
protein at 10ng
HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Strizzi L et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget 6:34071-86 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26460952) »
- Malchenko S et al. Cancer hallmarks in induced pluripotent cells: new insights. J Cell Physiol 225:390-3 (2010). WB ; Human . Read more (PubMed: 20568225) »