Product nameAnti-Nodal antibody [EP2058Y] - BSA and Azide free
See all Nodal primary antibodies
DescriptionRabbit monoclonal [EP2058Y] to Nodal - BSA and Azide free
This antibody detects Pro-Nodal and Nodal.
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Nodal aa 300-400 (C terminal). The exact sequence is proprietary.
- Recombinant nodal protein.
ab191550 is the carrier-free version of ab81287 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab191550 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
(Pro-Nodal) and 13 kDa (Nodal).
FunctionEssential for mesoderm formation and axial patterning during embryonic development.
Involvement in diseaseDefects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab191550 has not yet been referenced specifically in any publications.