RecombinantRabMAb

Recombinant Anti-Nodal antibody [EPR2057] (ab109317)

Submit a review Q&A (1)References (4)
Western blot - Anti-Nodal antibody [EPR2057] (ab109317)
  • Flow Cytometry - Anti-Nodal antibody [EPR2057] (ab109317)
  • Anti-Nodal antibody [EPR2057] (ab109317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2057] to Nodal
  • Suitable for: WB, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Nodal antibody [EPR2057]
    See all Nodal primary antibodies

  • Description

    Rabbit monoclonal [EPR2057] to Nodal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human Nodal aa 250-350. The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y and PC-12 cell lysates Flow Cyto: Permeabilized SH-SY5Y cells

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2057

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Essential for mesoderm formation and axial patterning during embryonic development.

    • Involvement in disease

      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.

    • Sequence similarities

      Belongs to the TGF-beta family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • HTX5 antibody
        • MGC138230 antibody
        • Nodal antibody
        • Nodal Growth Differentiation Factor antibody
        • nodal homolog (mouse) antibody
        • Nodal homolog antibody
        • Nodal, Mouse, Homolog antibody
        • NODAL_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Nodal antibody [EPR2057] (ab109317)
        Western blot - Anti-Nodal antibody [EPR2057] (ab109317)
        All lanes : Anti-Nodal antibody [EPR2057] (ab109317) at 1/1000 dilution

        Lane 1 : SH-SY5Y lysate
        Lane 2 : PC12 lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 40 kDa

      • Flow Cytometry - Anti-Nodal antibody [EPR2057] (ab109317)
        Flow Cytometry - Anti-Nodal antibody [EPR2057] (ab109317)
        Flow cytometric analysis of Nodal in permeabilized SH-SY5Y cells, using ab109317 (red) or a rabbit IgG (negative) (green).
      • Anti-Nodal antibody [EPR2057] (ab109317)
        Anti-Nodal antibody [EPR2057] (ab109317)

      References (4)

      Publishing research using ab109317? Please let us know so that we can cite the reference in this datasheet.

      ab109317 has been referenced in 4 publications.

      • Wei X  et al. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv 5:eaau7887 (2019). PubMed: 30891497
      • Li H  et al. Nodal induced by hypoxia exposure contributes to dacarbazine resistance and the maintenance of stemness in melanoma cancer stem-like cells. Oncol Rep 39:2855-2864 (2018). PubMed: 29693169
      • Xu C  et al. Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling. Nat Commun 7:13287 (2016). WB . PubMed: 27811843
      • Chen J  et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). WB ; Human . PubMed: 24465741

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      Question

      Hello. I am looking to test a few anti-Nodal antibodies for my project, but I have to target a specific region of the protein. I am wondering if you can provide some immunogen information. Specifically, are any of the antibodies below generated from a peptide upstream (N-terminal side) of residue XXX? Any information would be greatly appreciated. Thanks!

      Anti-Nodal antibodies:
      ab39242
      ab39953
      ab109317

      Read More
      Answer

      Thank you for contacting Abcam.

      The immunogen information you requested is listed below for each antibody:
      1. ab39242: immunogen is upstream of residue XXX.
      2. ab39953: immunogen is downstream of residue XXX.
      3. ab109317: immunogen is upstream of residue XXX.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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