Recombinant Anti-Nodal antibody [EPR2057] (ab109317)
![Western blot - Anti-Nodal antibody [EPR2057] (ab109317)](https://www.abcam.com/ps/products/109/ab109317/Images/ab109317-122090-anti-nodal-antibody-epr2057-western-blot.jpg "Western blot - Anti-Nodal antibody [EPR2057] (ab109317)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2057] to Nodal
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Nodal antibody [EPR2057]
See all Nodal primary antibodies -
Description
Rabbit monoclonal [EPR2057] to Nodal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y and PC-12 cell lysates Flow Cyt (intra)o: Permeabilized SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2057 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
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Function
Essential for mesoderm formation and axial patterning during embryonic development. -
Involvement in disease
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4838 Human
- Entrez Gene: 294503 Rat
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- Unigene: 370414 Human
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Alternative names
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
Images
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Western blot - Anti-Nodal antibody [EPR2057] (ab109317)All lanes : Anti-Nodal antibody [EPR2057] (ab109317) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : PC12 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Flow Cytometry (Intracellular) - Anti-Nodal antibody [EPR2057] (ab109317)](https://www.abcam.com/ps/products/109/ab109317/Images/ab109317-122089-anti-nodal-antibody-epr2057-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Nodal antibody [EPR2057] (ab109317)Intracellular flow cytometric analysis of Nodal in permeabilized SH-SY5Y cells, using ab109317 (red) or a rabbit IgG (negative) (green).
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![Anti-Nodal antibody [EPR2057] (ab109317)](https://www.abcam.com/ps/products/109/ab109317/Images/ab109317-3-benefits-of-recombinant-antibodies.png)
Anti-Nodal antibody [EPR2057] (ab109317)
Datasheets and documents
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Datasheet download
References (4)
ab109317 has been referenced in 4 publications.
- Wei X et al. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv 5:eaau7887 (2019). PubMed: 30891497
- Li H et al. Nodal induced by hypoxia exposure contributes to dacarbazine resistance and the maintenance of stemness in melanoma cancer stem-like cells. Oncol Rep 39:2855-2864 (2018). PubMed: 29693169
- Xu C et al. Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling. Nat Commun 7:13287 (2016). WB . PubMed: 27811843
- Chen J et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). WB ; Human . PubMed: 24465741
