Anti-Noelin antibody - C-terminal (ab151416)

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Overview

  • Product name

    Anti-Noelin antibody - C-terminal
    See all Noelin primary antibodies

  • Description

    Rabbit polyclonal to Noelin - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Xenopus laevis, Xenopus tropicalis

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 421-485 of Human Noelin (UniProt ID: Q99784).

  • Positive control

    • HepG2 cell lysate; mouse brain tissue lysate; NCI-N87 xenograft tissue; HeLa cells

  • General notes

    The immunogen was raised from noelin isoform 1 precursor(NP_055094)

Properties

Applications

Our Abpromise guarantee covers the use of ab151416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Anti-Noelin antibody - C-terminal (ab151416) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Anti-Noelin antibody - C-terminal (ab151416) at 1/1000 dilution + mouse brain tissue lysate at 50 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (ab151416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (ab151416)
    Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft labeling Noelin with ab151416 at 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (ab151416)
    Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (ab151416)
    Immunofluorescent analysis of methanol-fixed HeLa cells labeling Noelin with ab151416 at 1/500 dilution.
    Lower panel was co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Hill SE  et al. Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies. PLoS One 10:e0130888 (2015). Read more (PubMed: 26121352) »
See 1 Publication for this product

Publishing research using ab151416? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Thank you for your help. Do you know which isoform of the OLFM1 protein (Q99784) the 78-108 amino acids refer to? There are 4 different isoforms and therefore different sequences it could correspond to.

The same question apply to the ab151416 antibody.

Read More
Answer

Looking at the immunogen location for bothe antibodies , it is not involved in causing those isoforms, so it should recognize all isoforms.

Read More

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