Anti-Noelin antibody - C-terminal (ab151416)

Submit a review Q&A (1)References (1)
Western blot - Anti-Noelin antibody - C-terminal (ab151416)
  • Western blot - Anti-Noelin antibody - C-terminal (ab151416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (ab151416)
  • Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (ab151416)

Key features and details

  • Rabbit polyclonal to Noelin - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Noelin antibody - C-terminal
    See all Noelin primary antibodies

  • Description

    Rabbit polyclonal to Noelin - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 421-485 of Human Noelin (UniProt ID: Q99784).

  • Positive control

    • HepG2 cell lysate; mouse brain tissue lysate; NCI-N87 xenograft tissue; HeLa cells

  • General notes

    The immunogen was raised from noelin isoform 1 precursor(NP_055094)

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
All applications
Rat
Chicken
Xenopus laevis
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

Images

  • Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Anti-Noelin antibody - C-terminal (ab151416) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Western blot - Anti-Noelin antibody - C-terminal (ab151416)
    Anti-Noelin antibody - C-terminal (ab151416) at 1/1000 dilution + mouse brain tissue lysate at 50 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (ab151416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (ab151416)
    Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft labeling Noelin with ab151416 at 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (ab151416)
    Immunocytochemistry/ Immunofluorescence - Anti-Noelin antibody - C-terminal (ab151416)
    Immunofluorescent analysis of methanol-fixed HeLa cells labeling Noelin with ab151416 at 1/500 dilution.
    Lower panel was co-stained with Hoechst 33342.

References (1)

Publishing research using ab151416? Please let us know so that we can cite the reference in this datasheet.

ab151416 has been referenced in 1 publication.

  • Hill SE  et al. Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies. PLoS One 10:e0130888 (2015). PubMed: 26121352

Customer reviews and Q&As

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Submit a review Submit a question

Question

Thank you for your help. Do you know which isoform of the OLFM1 protein (Q99784) the 78-108 amino acids refer to? There are 4 different isoforms and therefore different sequences it could correspond to.

The same question apply to the ab151416 antibody.

Read More
Answer

Looking at the immunogen location for bothe antibodies , it is not involved in causing those isoforms, so it should recognize all isoforms.

Read More

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