Anti-Noelin antibody - C-terminal (ab151416)
Key features and details
- Rabbit polyclonal to Noelin - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Noelin antibody - C-terminal
See all Noelin primary antibodies -
Description
Rabbit polyclonal to Noelin - C-terminal -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB MouseHuman -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 421-485 of Human Noelin (UniProt ID: Q99784).
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Positive control
- HepG2 cell lysate; mouse brain tissue lysate; NCI-N87 xenograft tissue; HeLa cells
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General notes
The immunogen was raised from noelin isoform 1 precursor(NP_055094)Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Mouse
Human
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All applications |
Rat
Chicken
Xenopus laevis
Xenopus tropicalis
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Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Seems to play an important role in regulating the production of neural crest cells by the neural tube. -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 395535 Chicken
- Entrez Gene: 10439 Human
- Entrez Gene: 56177 Mouse
- Entrez Gene: 93667 Rat
- Entrez Gene: 398287 Xenopus laevis
- Entrez Gene: 100101729 Xenopus tropicalis
- Omim: 605366 Human
- SwissProt: Q9IAK4 Chicken
see all -
Alternative names
- AMY antibody
- NEUROBLASTOMA PROTEIN antibody
- Neuronal olfactomedin-related ER localized protein antibody
see all
Images
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Anti-Noelin antibody - C-terminal (ab151416) at 1/500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Anti-Noelin antibody - C-terminal (ab151416) at 1/1000 dilution + mouse brain tissue lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noelin antibody - C-terminal (ab151416)Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft labeling Noelin with ab151416 at 1/100 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling Noelin with ab151416 at 1/500 dilution.
Lower panel was co-stained with Hoechst 33342.
Protocols
References (1)
ab151416 has been referenced in 1 publication.
- Hill SE et al. Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies. PLoS One 10:e0130888 (2015). PubMed: 26121352