Anti-Noelin antibody - C-terminal (ab151416)
Key features and details
- Rabbit polyclonal to Noelin - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Noelin antibody - C-terminal
See all Noelin primary antibodies -
Description
Rabbit polyclonal to Noelin - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Xenopus laevis, Xenopus tropicalis -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 421-485 of Human Noelin (UniProt ID: Q99784).
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Positive control
- HepG2 cell lysate; mouse brain tissue lysate; NCI-N87 xenograft tissue; HeLa cells
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General notes
The immunogen was raised from noelin isoform 1 precursor(NP_055094)The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Seems to play an important role in regulating the production of neural crest cells by the neural tube. -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 395535 Chicken
- Entrez Gene: 10439 Human
- Entrez Gene: 56177 Mouse
- Entrez Gene: 93667 Rat
- Entrez Gene: 398287 Xenopus laevis
- Entrez Gene: 100101729 Xenopus tropicalis
- Omim: 605366 Human
- SwissProt: Q9IAK4 Chicken
see all -
Alternative names
- AMY antibody
- NEUROBLASTOMA PROTEIN antibody
- Neuronal olfactomedin-related ER localized protein antibody
see all
Images
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Anti-Noelin antibody - C-terminal (ab151416) at 1/500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Anti-Noelin antibody - C-terminal (ab151416) at 1/1000 dilution + mouse brain tissue lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft labeling Noelin with ab151416 at 1/100 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling Noelin with ab151416 at 1/500 dilution.
Lower panel was co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151416 has been referenced in 1 publication.
- Hill SE et al. Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies. PLoS One 10:e0130888 (2015). PubMed: 26121352