Key features and details
- Rabbit polyclonal to NOF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NOF antibody
DescriptionRabbit polyclonal to NOF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey, Orangutan
- WB: COLO 320 whole cell lysate; Mouse liver and kidney lysates. IHC-P: Human kidney and rectum tissues.
Previously labelled as MRPL49.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 19 kDa.|
|IHC-P||1/20 - 1/200.|
RelevanceMitochondria have their own translation system for production of 13 inner membrane proteins essential for oxidative phosphorylation. NOF is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for the HRD1 protein use in their respective 3' UTRs some of the same genomic sequence. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p.
- 39S ribosomal protein L49 mitochondrial antibody
- C11orf4 antibody
- Chromosome 11 open reading frame 4 antibody
All lanes : Anti-NOF antibody (ab235401) at 1/1000 dilution
Lane 1 : COLO 320 whole cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 19 kDa
Paraffin-embedded human kidney tissue stained for NOF using ab235401 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human rectum tissue stained for NOF using ab235401 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235401 has not yet been referenced specifically in any publications.