Key features and details
- Rabbit polyclonal to Nogo A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Nogo A antibody
See all Nogo A primary antibodies
DescriptionRabbit polyclonal to Nogo A
Specificityab62024 is specific for Nogo A and Nogo E.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Raised against a 19 amino acid synthetic peptide from near the centre of Human Nogo A (GenBank accession no. NP_065393).
- Human brain tissue and mouse brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab62024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2.5 µg/ml.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDevelopmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
Tissue specificityIsoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Sequence similaritiesContains 1 reticulon domain.
DomainThree regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
- Information by UniProt
- ASY antibody
- Foocen antibody
- Human NogoA antibody
Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/ml
Lane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG
Predicted band size: 130 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 155 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nogo A with Anti-Nogo A antibody (ab62024) at 5μg/ml.
ab62024, at 2.5µg/ml, staining Mouse Nogo A in brain tissue, by Immunohistochemistry
ICC/IF image of ab62024 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62024, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of NogoA in Mouse Brain cells using ab62024 at 20 ug/ml.
ab62024 has been referenced in 9 publications.
- Thau-Zuchman O et al. Brain Phospholipid Precursors Administered Post-Injury Reduce Tissue Damage and Improve Neurological Outcome in Experimental Traumatic Brain Injury. J Neurotrauma 36:25-42 (2019). PubMed: 29768974
- Mukherjee RN & Levy DL Reticulon 4a promotes exocytosis in mammalian cells. Mol Biol Cell 30:2349-2357 (2019). PubMed: 31318320
- Xie G et al. A Network Pharmacology Analysis to Explore the Effect of Astragali Radix-Radix Angelica Sinensis on Traumatic Brain Injury. Biomed Res Int 2018:3951783 (2018). PubMed: 30596090
- He R et al. Effect of fasudil on cognitive function following status convulsion in rats. Mol Med Rep 16:119-126 (2017). WB ; Rat . PubMed: 28534935
- Huang S et al. Electroacupuncture promotes axonal regeneration in rats with focal cerebral ischemia through the downregulation of Nogo-A/NgR/RhoA/ROCK signaling. Exp Ther Med 14:905-912 (2017). PubMed: 28810542
- Zhu B et al. Knockout of the Nogo-B Gene Attenuates Tumor Growth and Metastasis in Hepatocellular Carcinoma. Neoplasia 19:583-593 (2017). WB . PubMed: 28628795
- Travaglia A et al. Developmental changes in plasticity, synaptic, glia and connectivity protein levels in rat dorsal hippocampus. Neurobiol Learn Mem 135:125-138 (2016). PubMed: 27523749
- Rämö O et al. NOGO-A/RTN4A and NOGO-B/RTN4B are simultaneously expressed in epithelial, fibroblast and neuronal cells and maintain ER morphology. Sci Rep 6:35969 (2016). PubMed: 27786289
- Zhong J et al. Knockdown of NogoA prevents MPP+-induced neurotoxicity in PC12 cells via the mTOR/STAT3 signaling pathway. Mol Med Rep 13:1427-33 (2016). PubMed: 26648565