Product nameAnti-Nogo A antibody
See all Nogo A primary antibodies
DescriptionRabbit polyclonal to Nogo A
Specificityab62024 is specific for Nogo A and Nogo E.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Raised against a 19 amino acid synthetic peptide from near the centre of Human Nogo A (GenBank accession no. NP_065393).
- Human brain tissue and mouse brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab62024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2.5 µg/ml.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDevelopmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
Tissue specificityIsoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Sequence similaritiesContains 1 reticulon domain.
DomainThree regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
- Information by UniProt
- ASY antibody
- Foocen antibody
- Human NogoA antibody
Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/ml
Lane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti-rabbit IgG
Predicted band size: 130 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 155 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nogo A with Anti-Nogo A antibody (ab62024) at 5μg/ml.
ab62024, at 2.5µg/ml, staining Mouse Nogo A in brain tissue, by Immunohistochemistry
ICC/IF image of ab62024 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62024, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of NogoA in Mouse Brain cells using ab62024 at 20 ug/ml.
This product has been referenced in:
- Thau-Zuchman O et al. Brain Phospholipid Precursors Administered Post-Injury Reduce Tissue Damage and Improve Neurological Outcome in Experimental Traumatic Brain Injury. J Neurotrauma 36:25-42 (2019). Read more (PubMed: 29768974) »
- Mukherjee RN & Levy DL Reticulon 4a promotes exocytosis in mammalian cells. Mol Biol Cell 30:2349-2357 (2019). Read more (PubMed: 31318320) »