Anti-Nogo A+B antibody (ab47085)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Nogo A+B antibody
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DescriptionRabbit polyclonal to Nogo A+B
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Host speciesRabbit
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Specificityab47085 recognises Nogo A and Nogo B2 and B1
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Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, African green monkey
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Immunogen
Synthetic peptide, sequence taken from within amino acids 1-50 of human Nogo B.
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Positive control
- Human, mouse and rat brain tissue lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab47085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250. | |
| ICC/IF | 1/200. | |
| WB | 1/500 - 1/2000. Detects a band of approximately >200 kDa. |
Target
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RelevanceNOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (159460), and Nogo. Several isoforms (A-E) of NOGO exist.
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Cellular localizationEndoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
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Database links
- Entrez Gene: 57142 Human
- Entrez Gene: 68585 Mouse
- Entrez Gene: 83765 Rat
- Omim: 604475 Human
- SwissProt: Q9NQC3 Human
- SwissProt: Q99P72 Mouse
- SwissProt: Q9JK11 Rat
- Unigene: 704007 Human
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Alternative names
- Nogo A antibody
- Nogo antibody
- Nogo B antibody
see all
Images
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Western blot - Anti-Nogo A+B antibody (ab47085)All lanes : Anti-Nogo A+B antibody (ab47085) at 1/2000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Observed band size: >200,41,43,48-50 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (ab47085)This image is courtesy of an anonymous Abreviewab47085 staining Nogo A+B in African Green Monkey COS-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in PBS-BSA) for 1 hour at 23°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (ab47085)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunocytochemistry stainning for Nogo A+B in Cor1 neural stem cells from mouse using Rabbit polyclonal to Nogo A+B (ab47085; 1/200 incubated for 2h at RT); Immunoreactivity was detected in cell-body as well as in neurites.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhao H et al. Cell fate regulation by reticulon-4 in human prostate cancers. J Cell Physiol N/A:N/A (2018). Read more (PubMed: 30480803) »
- Grumati P et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . Read more (PubMed: 28617241) »
