Overview

  • Product name
    Anti-Nogo A+B antibody
  • Description
    Rabbit polyclonal to Nogo A+B
  • Host species
    Rabbit
  • Specificity
    ab47085 recognises Nogo A and Nogo B2 and B1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Synthetic peptide, sequence taken from within amino acids 1-50 of human Nogo B.

  • Positive control
    • Human, mouse and rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab47085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
ICC/IF 1/200.
WB 1/500 - 1/2000. Detects a band of approximately >200 kDa.

Target

  • Relevance
    NOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (159460), and Nogo. Several isoforms (A-E) of NOGO exist.
  • Cellular localization
    Endoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
  • Database links
    • Alternative names
      • Nogo A antibody
      • Nogo antibody
      • Nogo B antibody
      • NSP antibody
      • Reticulon 4 antibody
      • Reticulon 5 antibody
      • RTN x antibody
      • RTN4 antibody
      • RTN4A antibody
      • RTNX antibody
      see all

    Images

    • Western blot - Anti-Nogo A+B antibody (ab47085)
      Western blot - Anti-Nogo A+B antibody (ab47085)
      All lanes : Anti-Nogo A+B antibody (ab47085) at 1/2000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Mouse brain tissue lysate
      Lane 3 : Rat brain tissue lysate

      Observed band size: >200,41,43,48-50 kDa (why is the actual band size different from the predicted?)

    • Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (ab47085)
      Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (ab47085)This image is courtesy of an anonymous Abreview

      ab47085 staining Nogo A+B in African Green Monkey COS-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in PBS-BSA) for 1 hour at 23°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (ab47085)
      Immunocytochemistry/ Immunofluorescence - Anti-Nogo A+B antibody (ab47085)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
      Immunocytochemistry stainning for Nogo A+B in Cor1 neural stem cells from mouse using Rabbit polyclonal to Nogo A+B (ab47085; 1/200 incubated for 2h at RT); Immunoreactivity was detected in cell-body as well as in neurites.

    References

    This product has been referenced in:
    • Grumati P  et al. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife 6:N/A (2017). WB . Read more (PubMed: 28617241) »
    • Wen M  et al. The Value of Circulating Nogo-B for Evaluating Hepatic Functional Reserve in Patients with Cirrhosis. Dis Markers 2015:419124 (2015). Read more (PubMed: 26063954) »

    See all 3 Publications for this product

    Publishing research using ab47085? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    10 reviews or Q&A

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    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nogo A+B antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    3% formaldehyde, 0.02% glutaraldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Aug 03 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nogo A+B antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    African Green Monkey Cell (COS-7)
    Specification
    COS-7
    Permeabilization
    Yes - 0.5% Triton-X
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 25 2013

    Western blot

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Sample
    Mouse Cell lysate - whole cell (Mouse cardiac fibroblasts)
    Specification
    Mouse cardiac fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Western blot abreview for Anti-Nogo A+B antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HELA)
    Loading amount
    50 µg
    Specification
    HELA
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Sep 23 2011

    Western blot abreview for Anti-Nogo A+B antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Loading amount
    50 µg
    Specification
    MEF
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Sep 23 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nogo A+B antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEF)
    Specification
    MEF
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nogo A+B antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HELA)
    Specification
    HELA
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2011

    Western blot abreview for Anti-Nogo A+B antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Gastrocnemius muscle)
    Loading amount
    100 µg
    Specification
    Gastrocnemius muscle
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
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    Username

    Dr. Barney Bryson

    Verified customer

    Submitted Jun 02 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nogo A+B antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cor1 neural stem cells)
    Specification
    Cor1 neural stem cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Tween
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 10 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nogo A+B antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Forebrain: region of corpus callosum/striatum, cor)
    Specification
    Forebrain: region of corpus callosum/striatum, cor
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 26 2008

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