Overview

  • Product name

    Anti-Nogo A+B antibody
  • Description

    Rabbit polyclonal to Nogo A+B
  • Host species

    Rabbit
  • Specificity

    ab47085 recognises Nogo A and Nogo B2 and B1
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Synthetic peptide, sequence taken from within amino acids 1-50 of human Nogo B.

  • Positive control

    • Human, mouse and rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab47085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
ICC/IF 1/200.
WB 1/500 - 1/2000. Detects a band of approximately >200 kDa.

Target

  • Relevance

    NOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (159460), and Nogo. Several isoforms (A-E) of NOGO exist.
  • Cellular localization

    Endoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
  • Database links

  • Alternative names

    • Nogo A antibody
    • Nogo antibody
    • Nogo B antibody
    • NSP antibody
    • Reticulon 4 antibody
    • Reticulon 5 antibody
    • RTN x antibody
    • RTN4 antibody
    • RTN4A antibody
    • RTNX antibody
    see all

Images

  • All lanes : Anti-Nogo A+B antibody (ab47085) at 1/2000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Observed band size: >200,41,43,48-50 kDa
    why is the actual band size different from the predicted?

  • ab47085 staining Nogo A+B in African Green Monkey COS-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in PBS-BSA) for 1 hour at 23°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry stainning for Nogo A+B in Cor1 neural stem cells from mouse using Rabbit polyclonal to Nogo A+B (ab47085; 1/200 incubated for 2h at RT); Immunoreactivity was detected in cell-body as well as in neurites.

References

This product has been referenced in:

  • V'kovski P  et al. Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling. Elife 8:N/A (2019). Read more (PubMed: 30632963) »
  • Zhao H  et al. Cell fate regulation by reticulon-4 in human prostate cancers. J Cell Physiol N/A:N/A (2018). Read more (PubMed: 30480803) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
3% formaldehyde, 0.02% glutaraldehyde

Abcam user community

Verified customer

Submitted Aug 03 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
African Green Monkey Cell (COS-7)
Specification
COS-7
Permeabilization
Yes - 0.5% Triton-X
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 25 2013

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Cell lysate - whole cell (Mouse cardiac fibroblasts)
Specification
Mouse cardiac fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 29 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (HELA)
Loading amount
50 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 23 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Loading amount
50 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 23 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Specification
MEF
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 09 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 09 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Gastrocnemius muscle)
Loading amount
100 µg
Specification
Gastrocnemius muscle
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Barney Bryson

Verified customer

Submitted Jun 02 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cor1 neural stem cells)
Specification
Cor1 neural stem cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Tween
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Mr. Carl Hobbs

Verified customer

Submitted Feb 10 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Forebrain: region of corpus callosum/striatum, cor)
Specification
Forebrain: region of corpus callosum/striatum, cor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Mr. Carl Hobbs

Verified customer

Submitted Aug 26 2008

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