Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cor1 neural stem cells)
Specification
Cor1 neural stem cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Tween
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/TWEEN/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgGs
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
Please see my abreview ab6142 anti nestin for immunostaining details.
I have repeated this result three times and got the same result each time.
I have also tested ab32298 on Cor1 cells and on p.wax sections of mouse and rat adult brain and get the same result although the latter Ab is not as good as this one, imho.
Also, I have tested this Ab in rat/mouse pwax sections of embryos and I find that much higher dilution factors are required to obtain what I regard as specific staining.
I have repeated this result three times and got the same result each time.
I have also tested ab32298 on Cor1 cells and on p.wax sections of mouse and rat adult brain and get the same result although the latter Ab is not as good as this one, imho.
Also, I have tested this Ab in rat/mouse pwax sections of embryos and I find that much higher dilution factors are required to obtain what I regard as specific staining.
Mr. Carl Hobbs
Verified customer
Submitted Feb 10 2009