Application
Western blot
Sample
Human Cell lysate - whole cell (HELA)
Loading amount
50 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 36 kDa
Abcam user community
Verified customer
Submitted Sep 23 2011