Product nameAnti-Nogo antibody
See all Nogo primary antibodies
DescriptionRabbit polyclonal to Nogo
Specificityab32298 reacts with Nogo isoforms A and B (Foocen M1), and is predicted to react with the additional isoforms Nogo C and Foocen M2, due to sequence homology.
Tested applicationsSuitable for: IHC-FoFr, ICC, ICC/IF, WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig
- This antibody gave a positive signal in the following tissue lysates: Rat Hippocampus, Mouse Hippocampus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab32298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 170,45 kDa (predicted molecular weight: 126,39 kDa).Can be blocked with Rat Nogo peptide (ab32297).|
FunctionDevelopmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
Tissue specificityIsoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Sequence similaritiesContains 1 reticulon domain.
DomainThree regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
- Information by UniProt
- 1110020G17Rik antibody
- AA407876 antibody
- AA409940 antibody
All lanes : Anti-Nogo antibody (ab32298) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 126,39 kDa
Observed band size: 170,45 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 37 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Immunofluorescent staining for Nogo in rat brain cerebellum using Rabbit polyclonal to Nogo (ab32298; 1/300 incubated for 18h at RT); immunoreactivity was detected in most brain areas. The staining is quite diffuse on cell bodies, axons and dendrites. The picture was acquired using X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
Immunocytochemistry stainning for Nogo in Cor1 neural stem cells from mouse using Rabbit polyclonal to Nogo (ab32298; 1/200 incubated for 1h at RT); Immunoreactivity was detected in cell-body as well as in neurites.
ab32298 staining Nogo in human SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, blocked with 10% serum for 20 minutes at 24°C, then incubated with ab32298 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
This product has been referenced in:
- Vanguilder HD et al. Hippocampal expression of myelin-associated inhibitors is induced with age-related cognitive decline and correlates with deficits of spatial learning and memory. J Neurochem 121:77-98 (2012). WB ; Rat . Read more (PubMed: 22269040) »