Overview

  • Product name

    Anti-Nogo antibody [EPR12266]
    See all Nogo primary antibodies
  • Description

    Rabbit monoclonal [EPR12266] to Nogo
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nogo aa 1150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NQC3

  • Positive control

    • U87-MG, SH-SY5Y, and HeLa cell lysates; U87-MG cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 40-190 kDa (predicted molecular weight: 129 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
    • Tissue specificity

      Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
    • Sequence similarities

      Contains 1 reticulon domain.
    • Domain

      Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
    • Cellular localization

      Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1110020G17Rik antibody
      • AA407876 antibody
      • AA409940 antibody
      • AA960376 antibody
      • ASY antibody
      • C130026I10Rik antibody
      • Foocen antibody
      • Glut4 vesicle 20 kDa protein antibody
      • Human NogoA antibody
      • Kiaa0886 antibody
      • KIAA4153 antibody
      • MGC116054 antibody
      • MGC139261 antibody
      • mKIAA0886 antibody
      • mKIAA4153 antibody
      • My043 protein antibody
      • Nbla00271 antibody
      • Nbla10545 antibody
      • Neurite growth inhibitor 220 antibody
      • Neurite Growth Inhibitor 220, included antibody
      • Neurite outgrowth inhibitor antibody
      • Neuroendocrine-specific protein antibody
      • Neuroendocrine-specific protein C homolog antibody
      • NI-250 antibody
      • NI220/250 antibody
      • Nogo A antibody
      • NOGO antibody
      • Nogo B antibody
      • Nogo C antibody
      • Nogo protein antibody
      • NOGOC antibody
      • NSP antibody
      • NSP-CL antibody
      • rat N antibody
      • Reticulon 4 antibody
      • Reticulon 5 antibody
      • Reticulon-4 antibody
      • Reticulon-5 antibody
      • RTN X antibody
      • RTN-x antibody
      • Rtn4 antibody
      • Rtn4 reticulon 4 antibody
      • RTN4-A antibody
      • RTN4-B1 antibody
      • RTN4-B2 antibody
      • RTN4-C antibody
      • RTN4_HUMAN antibody
      • Vp20 antibody
      see all

    Images

    • Flow Cytometric analysis of permeabilized U87-MG cells labeling Nogo with ab177953 at 1/100 dilution  (red) or a rabbit IgG (negative) (green).

    • All lanes : Anti-Nogo antibody [EPR12266] (ab177953) at 1/1000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 129 kDa

    References

    ab177953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177953.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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