Recombinant Anti-Nogo Receptor antibody [EPR11159] (ab184556)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11159] to Nogo Receptor
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Nogo Receptor antibody [EPR11159]
See all Nogo Receptor primary antibodies -
Description
Rabbit monoclonal [EPR11159] to Nogo Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- SH-SY5Y, 293 and HepG2 cell lysates. Neuro-2a and C6 whole cell lysates. Mouse brain and hippocampus tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11159 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 51 kDa).
|
|
IP |
1/50 - 1/70.
|
Notes |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 51 kDa). |
IP
1/50 - 1/70. |
Target
-
Relevance
Nogo Receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. It is also a receptor for Nogo A (RTN4), OMG and MAG. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 65078 Human
- Entrez Gene: 65079 Mouse
- Entrez Gene: 113912 Rat
- Omim: 605566 Human
- SwissProt: Q9BZR6 Human
- SwissProt: Q99PI8 Mouse
- SwissProt: Q99M75 Rat
-
Alternative names
- NgR antibody
- Nogo 66 receptor antibody
- Nogo receptor antibody
see all
Images
-
All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/5000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse hippocampus tissue lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa -
All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/50000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/10000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
Western blot analysis of Nogo Receptor in HepG2 cell lysate immunoprecipitated with ab184556 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (4)
ab184556 has been referenced in 4 publications.
- Rodriguez CM et al. Targeting RTN4/NoGo-Receptor reduces levels of ALS protein ataxin-2. Cell Rep 41:111505 (2022). PubMed: 36288715
- Liu H et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). WB ; Rat . PubMed: 33356694
- Zeng K et al. RNAi targeting Nogo Receptor enhanced survival and proliferation of murine retinal ganglion cells during N-methyl-D-aspartate-induced optic nerve crush. Oncotarget 8:65009-65021 (2017). PubMed: 29029408
- Guo X & Liu X Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats. Mol Med Rep 16:2030-2036 (2017). PubMed: 28656312