Overview

  • Product name

    Anti-Nogo Receptor antibody [EPR11159]
    See all Nogo Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR11159] to Nogo Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Nogo Receptor aa 350-450. The exact sequence is proprietary.
    Database link: Q9BZR6

  • Positive control

    • SH-SY5Y, 293 and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 51 kDa).
IP 1/50 - 1/70.

Target

  • Relevance

    Nogo Receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. It is also a receptor for Nogo A (RTN4), OMG and MAG.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • NgR antibody
    • Nogo 66 receptor antibody
    • Nogo receptor antibody
    • Nogor antibody
    • Reticulon 4 receptor antibody
    • Rtn4r antibody
    • UNQ330/PRO526 antibody
    see all

Images

  • All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/50000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/10000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 66 kDa why is the actual band size different from the predicted?

  • Western blot analysis of Nogo Receptor in HepG2 cell lysate immunoprecipitated with ab184556 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

This product has been referenced in:

  • Zeng K  et al. RNAi targeting Nogo Receptor enhanced survival and proliferation of murine retinal ganglion cells during N-methyl-D-aspartate-induced optic nerve crush. Oncotarget 8:65009-65021 (2017). Read more (PubMed: 29029408) »
  • Guo X & Liu X Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats. Mol Med Rep 16:2030-2036 (2017). Read more (PubMed: 28656312) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184556.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up