Anti-NOL12 antibody (ab111704)
Key features and details
- Rabbit polyclonal to NOL12
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NOL12 antibody -
Description
Rabbit polyclonal to NOL12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment within Human NOL12 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UGY1 -
Positive control
- Raji whole cell lysate; A431 cells; Mouse brain whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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|
ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
ICC/IF
1/100 - 1/500. |
Target
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Function
May bind to 28S rRNA. -
Sequence similarities
Belongs to the RRP17 family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 79159 Human
- Entrez Gene: 97961 Mouse
- Entrez Gene: 362955 Rat
- SwissProt: Q9UGY1 Human
- SwissProt: Q8BG17 Mouse
- SwissProt: Q5D1Z3 Rat
- Unigene: 632778 Human
- Unigene: 157216 Mouse
see all -
Alternative names
- C78541 antibody
- dJ37E16.7 antibody
- FLJ34609 antibody
see all
Images
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Anti-NOL12 antibody (ab111704) at 1/1000 dilution + Raji whole cells lysate at 30 µg
Predicted band size: 25 kDa
Gel concentration: 12% -
Anti-NOL12 antibody (ab111704) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 25 kDa
Gel concentration: 12% -
Immunofluorescence analysis of paraformaldehyde-fixed A431, using b111704 at 1/200 dilution. Bottom image shows the same sample counterstained with Hoechst 33342
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111704 has not yet been referenced specifically in any publications.