Anti-NOL12 antibody (ab111704)
Key features and details
- Rabbit polyclonal to NOL12
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOL12 antibody -
Description
Rabbit polyclonal to NOL12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 161 of human NOL12 (Q9UGY1)
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Positive control
- Raji whole cell lysate; A431 cells; Mouse brain whole cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 25 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
May bind to 28S rRNA. -
Sequence similarities
Belongs to the RRP17 family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 79159 Human
- Entrez Gene: 97961 Mouse
- Entrez Gene: 362955 Rat
- SwissProt: Q9UGY1 Human
- SwissProt: Q8BG17 Mouse
- SwissProt: Q5D1Z3 Rat
- Unigene: 632778 Human
- Unigene: 157216 Mouse
see all -
Alternative names
- C78541 antibody
- dJ37E16.7 antibody
- FLJ34609 antibody
see all
Images
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Western blot - Anti-NOL12 antibody (ab111704)Anti-NOL12 antibody (ab111704) at 1/1000 dilution + Raji whole cells lysate at 30 µg
Predicted band size: 25 kDa
Gel concentration: 12% -
Western blot - Anti-NOL12 antibody (ab111704)Anti-NOL12 antibody (ab111704) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 25 kDa
Gel concentration: 12% -
Immunocytochemistry/ Immunofluorescence - Anti-NOL12 antibody (ab111704)Immunofluorescence analysis of paraformaldehyde-fixed A431, using b111704 at 1/200 dilution. Bottom image shows the same sample counterstained with Hoechst 33342
Protocols
References (0)
ab111704 has not yet been referenced specifically in any publications.
