Overview

  • Product name

  • Description

    Rabbit polyclonal to NOL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human NOL3 aa 1-197. (AAH12798)

  • Positive control

    • WB: HeLa lysate ICC/IF: HeLa cells IHC-P: HeLa xenograft; muscle tissue
  • General notes

     This product was previously labelled as Apoptosis repressor with CARD

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Isoform 1 may be involved in RNA splicing.
    Isoform 2 may inhibit apoptosis.
  • Tissue specificity

    Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
  • Sequence similarities

    Contains 1 CARD domain.
  • Cellular localization

    Cytoplasm and Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis repressor with CARD antibody
    • ARC antibody
    • Muscle enriched cytoplasmic protein antibody
    • Muscle-enriched cytoplasmic protein antibody
    • MYC antibody
    • MYP antibody
    • Nol3 antibody
    • NOL3_HUMAN antibody
    • NOP antibody
    • Nop30 antibody
    • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
    • Nucleolar protein 3 antibody
    • Nucleolar protein of 30 kDa antibody
    see all

Images

  • Anti-NOL3 antibody (ab126238) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE
  • Confocal immunofluorescence analysis of ab126238, at a 1/500 dilution, staining NOL3 in paraformaldehyde fixed HeLa cells (Green). Alpha-tubulin filaments were labeled with another antibody (Red) at a 1/2000 dilution.

  • ab126238, at a 1/500 dilution, staining NOL3 in paraffin embedded muscle tissue by Immunohistochemistry.

  • ab126238, at 1/500 dilution, staining Human NOL3 in paraffin embedded HeLa xenograft by Immunohistochemistry.

References

This product has been referenced in:

  • Xu T  et al. ARC regulates programmed necrosis and myocardial ischemia/reperfusion injury through the inhibition of mPTP opening. Redox Biol 20:414-426 (2019). Read more (PubMed: 30415165) »
  • Li J  et al. SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain. Nat Commun 5:5177 (2014). Read more (PubMed: 25295779) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you very much for contacting us with your enquiry, and I'm very sorry for the delay getting back to you.

I've received the fixation and permeabilization protocol from the lab:

1.Add 500µl of 4% paraformaldehyde/PBS into each well, leave for 15mins at room temperature
2.Remove the paraformaldehyde and wash coverslips by PBS for 3mins, twice.
3.Add 500µl of 0.1% Triton X-100 in PBS for 4 mins.
4.Wash cells by PBS for 3mins, twice.

Both ab126238 and ab137816 work well in ICC with human cells, and they are both fully guaranteed to work in this assay. We don't have much feedback on these antibodies, and they haven't been tested side-by-side. Based on the data, it's difficult to say which is better. They have slightly different specificities (ab126238 for amino acids 1-197, and ab137816 for amino acids 138-192), other than that they are very similar. I am sorry that I don't have a particular suggestion.

I hope that this information will be useful, but if you have further questions please let me know and I'll be happy to help.

Read More

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