Key features and details
- Rabbit polyclonal to NOL3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NOL3 antibody
See all NOL3 primary antibodies
DescriptionRabbit polyclonal to NOL3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human NOL3 aa 1-197. (AAH12798)
- WB: HeLa lysate ICC/IF: HeLa cells IHC-P: HeLa xenograft; muscle tissue
This product was previously labelled as Apoptosis repressor with CARD
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 24 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionIsoform 1 may be involved in RNA splicing.
Isoform 2 may inhibit apoptosis.
Tissue specificityHighly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
Sequence similaritiesContains 1 CARD domain.
Cellular localizationCytoplasm and Nucleus > nucleolus.
- Information by UniProt
- Apoptosis repressor with CARD antibody
- ARC antibody
- Muscle enriched cytoplasmic protein antibody
Anti-NOL3 antibody (ab126238) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS PAGE
Confocal immunofluorescence analysis of ab126238, at a 1/500 dilution, staining NOL3 in paraformaldehyde fixed HeLa cells (Green). Alpha-tubulin filaments were labeled with another antibody (Red) at a 1/2000 dilution.
ab126238, at a 1/500 dilution, staining NOL3 in paraffin embedded muscle tissue by Immunohistochemistry.
ab126238, at 1/500 dilution, staining Human NOL3 in paraffin embedded HeLa xenograft by Immunohistochemistry.
ab126238 has been referenced in 2 publications.
- Xu T et al. ARC regulates programmed necrosis and myocardial ischemia/reperfusion injury through the inhibition of mPTP opening. Redox Biol 20:414-426 (2019). PubMed: 30415165
- Li J et al. SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain. Nat Commun 5:5177 (2014). PubMed: 25295779