Anti-NOL3 antibody (ab2002)

Submit a review Q&A (2)References (1)
Western blot - Anti-NOL3 antibody (ab2002)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)

Key features and details

  • Rabbit polyclonal to NOL3
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NOL3 antibody
    See all NOL3 primary antibodies

  • Description

    Rabbit polyclonal to NOL3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human NOL3 aa 191-208 (C terminal).
    Sequence:

    PDPEPEPDFEERDESEDS


    (Peptide available as ab8371)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa whole cell lysate

  • General notes

    NOL3 domain

     This product was previously labelled as Apoptosis repressor with CARD

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
WB
Notes
  • Application notes
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Isoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.

    • Tissue specificity

      Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

    • Sequence similarities

      Contains 1 CARD domain.

    • Cellular localization

      Cytoplasm and Nucleus > nucleolus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Apoptosis repressor with CARD antibody
        • ARC antibody
        • Muscle enriched cytoplasmic protein antibody
        • Muscle-enriched cytoplasmic protein antibody
        • MYC antibody
        • MYP antibody
        • Nol3 antibody
        • NOL3_HUMAN antibody
        • NOP antibody
        • Nop30 antibody
        • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
        • Nucleolar protein 3 antibody
        • Nucleolar protein of 30 kDa antibody
        see all

      Images

      • Western blot - Anti-NOL3 antibody (ab2002)
        Western blot - Anti-NOL3 antibody (ab2002)
        All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution

        Lane 1 : HeLa whole cell lysate
        Lane 2 : KB whole cell lysate
        Lane 3 : A549 whole cell lysate

        Observed band size: 25 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)

        Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL3 antibody (ab2002)

        Immunohistochemistry of NOL3  in skeletal muscle with Anti-NOL3  (ab2002) at 5 µg/ml.

      References (1)

      Publishing research using ab2002? Please let us know so that we can cite the reference in this datasheet.

      ab2002 has been referenced in 1 publication.

      • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . PubMed: 24344197

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      How are you?

      I thought this may interest Abcam.

      Please see below the response from our customer:

      I have a proposal for your supplier. I am more than happy to write a review in return for a new supply. We have performed both IHC and WB with this antibody and we have fantastic results. By comparison, the IHC images given in your data sheet is not convincing. It look like H&E staining rather than and IHC staining. If your supplier is interested, I am more than happy for further talks.

      I look forward to receiving your response!

      Kind regards,

      Read More
      Answer

      Thank you for forwarding this enquiry.

      Reviewing the IHC images from the datasheet, I understood the customers concerns and contacted the testing laboratory.The images were provided tothem by a collaborator who uses red dyes for detection. In the human heart you can see darker pink/red areas of cytoplasmic staining. However, unfortunately it is difficult to tell with the skeletal muscle tissue. They are going to retest the antibody inIHC with standard DAB detection.

      I am sorry we are not able to offer anything further for this customer on this occasion as the antibody is already tested and covered by our guarantee in IHC-P. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet and the customer may like to consider this in return for Abpoint rewards. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contactme again with any other questions.

      Read More

      Question

      Dear Tech support,

      How are you?

      I have a question from a customer and may require your assistance on this please; please see below:

      I am planning to use one of your antibodies (ab 2002-100), nucleolar protein 30 (NOP 30). As you know, NOP30 is the product of the NOL3 gene. Due to alternative splicing, NOL3 produces two protein products, ARC and NOP 30. In the literature, NOL3, ARC and NOP30 are considered synonyms. Your datasheet says the same. Given that the C-terminal region of ARC is proline/glutamic acid (P/E) rich and that of NOP30 is serine/arginine (S/R) rich, I am not sure if it is appropriate to consider both as synonyms. My question, therefore, is what will I be detecting? Is it NOP30 or ARC?

      Please advise!

      Kind regards,

      Read More
      Answer

      Thank you for your enquiry.

      Based on the customers question I would define ab2002Anti-Apoptosis repressor with CARD antibodyas specific to ARC rather than NOP30. Looking at the two isoforms on UniProt the antibody is specific to isoform 2 as listed under O60936.

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

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