Anti-NOL3 antibody (ab2002)
Key features and details
- Rabbit polyclonal to NOL3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOL3 antibody
See all NOL3 primary antibodies -
Description
Rabbit polyclonal to NOL3 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human NOL3 aa 191-208 (C terminal).
Sequence:PDPEPEPDFEERDESEDS
(Peptide available asab8371) -
Positive control
- HeLa whole cell lysate
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General notes
NOL3 domain
This product was previously labelled as Apoptosis repressor with CARD
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
IHC-P | ||
WB |
Notes |
---|
WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Isoform 1 may be involved in RNA splicing.
Isoform 2 may inhibit apoptosis. -
Tissue specificity
Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas. -
Sequence similarities
Contains 1 CARD domain. -
Cellular localization
Cytoplasm and Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 8996 Human
- Omim: 605235 Human
- SwissProt: O60936 Human
- Unigene: 513667 Human
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Alternative names
- Apoptosis repressor with CARD antibody
- ARC antibody
- Muscle enriched cytoplasmic protein antibody
see all
Images
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All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : KB whole cell lysate
Lane 3 : A549 whole cell lysate
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.
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Immunohistochemistry of NOL3 in skeletal muscle with Anti-NOL3 (ab2002) at 5 µg/ml.
Protocols
Datasheets and documents
References (1)
ab2002 has been referenced in 1 publication.
- Razorenova OV et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . PubMed: 24344197