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    nol3-antibody-ab2003.pdf

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Cell Biology Apoptosis Intracellular Caspases etc CARD Family
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Anti-NOL3 antibody (ab2003)

  • Datasheet
Submit a review Q&A (1)References (1)

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Western blot - Anti-NOL3 antibody (ab2003)

    Key features and details

    • Rabbit polyclonal to NOL3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-NOL3 antibody
      See all NOL3 primary antibodies
    • Description

      Rabbit polyclonal to NOL3
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human NOL3 aa 2-18 (N terminal).
      Sequence:

      GNAQERPSETIDRERKR


      (Peptide available as ab8372)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • HeLa whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Caspases etc
      • CARD Family

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human NOL3 protein (ab131692)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2003 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500.
    Notes
    WB
    1/500.

    Target

    • Function

      Isoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.
    • Tissue specificity

      Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
    • Sequence similarities

      Contains 1 CARD domain.
    • Cellular localization

      Cytoplasm and Nucleus > nucleolus.
    • Target information above from: UniProt accession O60936 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8996 Human
      • Omim: 605235 Human
      • SwissProt: O60936 Human
      • Unigene: 513667 Human
      • Alternative names

        • Apoptosis repressor with CARD antibody
        • ARC antibody
        • Muscle enriched cytoplasmic protein antibody
        • Muscle-enriched cytoplasmic protein antibody
        • MYC antibody
        • MYP antibody
        • Nol3 antibody
        • NOL3_HUMAN antibody
        • NOP antibody
        • Nop30 antibody
        • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
        • Nucleolar protein 3 antibody
        • Nucleolar protein of 30 kDa antibody
        see all

      Images

      • Western blot - Anti-NOL3 antibody (ab2003)
        Western blot - Anti-NOL3 antibody (ab2003)
        Anti-NOL3 antibody (ab2003) at 1/500 dilution + HeLa whole cell lysate

        Observed band size: 25 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (1)

      Publishing research using ab2003? Please let us know so that we can cite the reference in this datasheet.

      ab2003 has been referenced in 1 publication.

      • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB ; Human . PubMed: 24344197

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I`m wondering if I could use your ARC(NT)- antibody for immunofluorescence staining of tissue sections and second, I`m wondering if you get any cross reactivity on your Western blot? Thank you,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 02 2001

      Answer

      The anti-ARC (NT) was affinity purified antibody using immunogenic peptide. It was shown very clean in Western bloting using HeLa, mouse and rat tissue lysates. We havn't test immunohistochemistry with this antibody. It worth to try.

      Read More

      Abcam Scientific Support

      Answered on Feb 02 2001

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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