Key features and details
- Rabbit polyclonal to NOL5A - C-terminal
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NOL5A antibody - C-terminal
See all NOL5A primary antibodies
DescriptionRabbit polyclonal to NOL5A - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human NOL5A (C terminal). The exact sequence is proprietary.
Database link: O00567
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
This product was previously labelled as NOL5A
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 66 kDa.|
RelevanceNOL5A is similar in sequence to Nop56p, a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Like Nop56p, NOL5A is found in the nucleolus and is though to be required for 60S ribosomal subunit biogenesis. Multiple transcript variants encoding several isoforms have been identified for this gene, but the full-length nature of most of them is not yet determined.
Cellular localizationNuclear; nucleolus
- NOL5A antibody
- NOP56 antibody
- NOP56 ribonucleoprotein antibody
All lanes : Anti-NOL5A antibody - C-terminal (ab229497) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 66 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human breast carcinoma tissue stained for NOL5A using ab229497 at 1/500 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL5A (green) using ab229497 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
NOL5A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229497. Western blot was performed from the immunoprecipitate using ab229497. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229497 in HEK-293T whole cell extract.
Lane 2: ab229497 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229497 has not yet been referenced specifically in any publications.