Overview

  • Product name

    Anti-NOL5A antibody - C-terminal
    See all NOL5A primary antibodies
  • Description

    Rabbit polyclonal to NOL5A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human NOL5A (C terminal). The exact sequence is proprietary.
    Database link: O00567

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as NOL5A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.

Target

  • Relevance

    NOL5A is similar in sequence to Nop56p, a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Like Nop56p, NOL5A is found in the nucleolus and is though to be required for 60S ribosomal subunit biogenesis. Multiple transcript variants encoding several isoforms have been identified for this gene, but the full-length nature of most of them is not yet determined.
  • Cellular localization

    Nuclear; nucleolus
  • Database links

  • Alternative names

    • NOL5A antibody
    • NOP56 antibody
    • NOP56 ribonucleoprotein antibody
    • NOP56 ribonucleoprotein homolog (yeast) antibody
    • NOP56 ribonucleoprotein homolog antibody
    • Nucleolar protein 56 antibody
    • Nucleolar protein 5A (56kD with KKE/D repeat) antibody
    • Nucleolar protein 5A antibody
    • Nucleolar protein Nop56 antibody
    • RP4-686C3.1 antibody
    • SCA36 antibody
    see all

Images

  • All lanes : Anti-NOL5A antibody - C-terminal (ab229497) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 66 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human breast carcinoma tissue stained for NOL5A using ab229497 at 1/500 dilution in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL5A (green) using ab229497 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • NOL5A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229497. Western blot was performed from the immunoprecipitate using ab229497. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG instead of ab229497 in HEK-293T whole cell extract. 
    Lane 2: ab229497 IP in HEK-293T whole cell extract. 

References

ab229497 has not yet been referenced specifically in any publications.

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