Product nameAnti-NOL5A antibody - C-terminal
See all NOL5A primary antibodies
DescriptionRabbit polyclonal to NOL5A - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human NOL5A (C terminal). The exact sequence is proprietary.
Database link: O00567
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
This product was previously labelled as NOL5A
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 66 kDa.|
RelevanceNOL5A is similar in sequence to Nop56p, a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Like Nop56p, NOL5A is found in the nucleolus and is though to be required for 60S ribosomal subunit biogenesis. Multiple transcript variants encoding several isoforms have been identified for this gene, but the full-length nature of most of them is not yet determined.
Cellular localizationNuclear; nucleolus
- NOL5A antibody
- NOP56 antibody
- NOP56 ribonucleoprotein antibody
All lanes : Anti-NOL5A antibody - C-terminal (ab229497) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 66 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human breast carcinoma tissue stained for NOL5A using ab229497 at 1/500 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL5A (green) using ab229497 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
NOL5A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229497. Western blot was performed from the immunoprecipitate using ab229497. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229497 in HEK-293T whole cell extract.
Lane 2: ab229497 IP in HEK-293T whole cell extract.
ab229497 has not yet been referenced specifically in any publications.