Overview

  • Product name

  • Description

    Rabbit polyclonal to NOL6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human NOL6 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H6R4

  • Positive control

    • WB: U-87 MG and mouse brain whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 128 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    NOL6 is a nucleolar RNA associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts.
  • Cellular localization

    Nucleus; nucleolus. Localizes to condensed chromosomes in mitosis
  • Database links

  • Alternative names

    • bA311H10.1 antibody
    • FLJ21959 antibody
    • MGC14896 antibody
    • MGC14921 antibody
    • MGC20838 antibody
    • NRAP antibody
    • Nucleolar protein 6 antibody
    • Nucleolar protein family 6 (RNA associated) antibody
    • Nucleolar RNA associated protein antibody
    • UTP22 antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NOL6 (green) using ab228836 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Counterstain: Hoechst 33342 (blue) and alpha tubulin filaments (red).

  • Anti-NOL6 antibody (ab228836) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 128 kDa



    5% SDS-PAGE

  • Anti-NOL6 antibody (ab228836) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 128 kDa



    12% SDS-PAGE

References

ab228836 has not yet been referenced specifically in any publications.

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