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Rabbit polyclonal to NOL8
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 200 and 250 of human NOL8 (NP_060418.4).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
Contains 1 RRM (RNA recognition motif) domain.
Nucleus > nucleolus.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL8 antibody (ab95898)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling NOL8 with ab95898 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-NOL8 antibody (ab95898)
All lanes : Anti-NOL8 antibody (ab95898) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 132 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-NOL8 antibody (ab95898)
Detection of human NOL8 by Western Blot of Immunoprecipitate.
ab95898 at 0.1µg/ml staining NOL8 in HeLa cell lysate immunoprecipitated using ab95898 at 3µg/mg (1mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"