Key features and details
- Rabbit polyclonal to NOL8 - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NOL8 antibody - N-terminal
DescriptionRabbit polyclonal to NOL8 - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human NOL8 (N terminal). The exact sequence is proprietary.
Database link: Q76FK4
- WB: HeLa whole cell extract. IP: HeLa whole cell extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 132 kDa.|
FunctionPlays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
Tissue specificityExpressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- C9orf34 antibody
- NOL8 antibody
- NOL8_HUMAN antibody
Anti-NOL8 antibody - N-terminal (ab229554) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 132 kDa
7.5% SDS-PAGE gel.
NOL8 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229544. Western blot was performed from the immunoprecipitate using ab229544.
Lane 1: HeLa whole cell extract (Input)
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab229554 IP in HeLa whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229554 has not yet been referenced specifically in any publications.