Overview

  • Product name

    Anti-NOL8 antibody - N-terminal
  • Description

    Rabbit polyclonal to NOL8 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NOL8 (N terminal). The exact sequence is proprietary.
    Database link: Q76FK4

  • Positive control

    • WB: HeLa whole cell extract. IP: HeLa whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 132 kDa.

Target

  • Function

    Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
  • Tissue specificity

    Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C9orf34 antibody
    • NOL8 antibody
    • NOL8_HUMAN antibody
    • NOP132 antibody
    • Nucleolar protein 8 antibody
    • Nucleolar protein Nop132 antibody
    see all

Images

  • Anti-NOL8 antibody - N-terminal (ab229554) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 132 kDa



    7.5% SDS-PAGE gel.

  • NOL8 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229544. Western blot was performed from the immunoprecipitate using ab229544.

    Lane 1: HeLa whole cell extract (Input)

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab229554 IP in HeLa whole cell extract.

References

ab229554 has not yet been referenced specifically in any publications.

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