Overview

  • Product name

    Anti-NOLC1 antibody [EPR14896]
    See all NOLC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14896] to NOLC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human NOLC1 aa 50-150. The exact sequence is proprietary.
    Database link: Q14978

  • Positive control

    • K562 and HeLa cell lysates, Human prostate tissue, K562 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 74 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/500.
Flow Cyt 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
  • Sequence similarities

    Contains 1 LisH domain.
  • Post-translational
    modifications

    Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDK1 kinase phosphorylates p130 at the M-phase.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • 140 kDa nucleolar phosphoprotein antibody
    • HCV NS5A trans regulated protein 13 antibody
    • HCV NS5A transactivated protein 13 antibody
    • HCV NS5A-transactivated protein 13 antibody
    • Hepatitis C virus NS5A transactivated protein 13 antibody
    • Hepatitis C virus NS5A-transactivated protein 13 antibody
    • KIAA0035 antibody
    • NOLC 1 antibody
    • NOLC1 antibody
    • NOLC1_HUMAN antibody
    • NOPP 130 antibody
    • NOPP 140 antibody
    • NOPP130 antibody
    • Nopp140 antibody
    • NS5ATP13 antibody
    • Nucleolar 130 kDa protein antibody
    • Nucleolar and coiled body phosphoprotein 1 antibody
    • Nucleolar and coiled body phosphprotein 1 antibody
    • Nucleolar and coiled-body phosphoprotein 1 antibody
    • Nucleolar phosphoprotein p130 antibody
    • Nucleolar protein p130 antibody
    • P130 antibody
    see all

Images

  • Immunofluorescence staining of -20℃ Acetone fixed K562 cells labeling NOLC1 using ab184550 at a 1/500 dilution (Red). Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution. Counterstained with Dapi (blue).

  • Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling NOLC1 with ab184550 at a 1/300 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling NOLC1 at a 1/100 dilution, counterstained with Hematoxylin.

  • All lanes : Anti-NOLC1 antibody [EPR14896] (ab184550) at 1/50000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa

References

This product has been referenced in:

  • Yuan F  et al. Nucleolar TRF2 attenuated nucleolus stress-induced HCC cell-cycle arrest by altering rRNA synthesis. Cell Death Dis 9:518 (2018). Read more (PubMed: 29725012) »
  • Yuan F  et al. Nucleolar and coiled-body phosphoprotein 1 (NOLC1) regulates the nucleolar retention of TRF2. Cell Death Discov 3:17043 (2017). Read more (PubMed: 28875039) »
See all 2 Publications for this product

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