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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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RecombinantRabMAb

Recombinant Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

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  • Certificate of Compliance
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Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
  • Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
  • Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22933-9] to non-muscle Myosin IIA
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant Human non-muscle Myosin IIA protein (Tagged) (ab226278)

View more associated products

Overview

  • Product name

    Anti-non-muscle Myosin IIA antibody [EPR22933-9]
    See all non-muscle Myosin IIA primary antibodies
  • Description

    Rabbit monoclonal [EPR22933-9] to non-muscle Myosin IIA
  • Host species

    Rabbit
  • Specificity

    IHC is not recommended for human.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse non-muscle Myosin IIA aa 250-350. The exact sequence is proprietary.
    Database link: Q8VDD5

  • Positive control

    • WB: Human liver and lung tissue lysates; mouse lung tissue lysate; rat spleen and lung tissue lysates; Neuro-2a, HEK-293T and HeLa whole cell lysates; IP: NIH/3T3 whole cell lysate; IHC-P: Mouse liver tissue; rat liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22933-9
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

Associated products

  • Alternative Versions

    • Anti-non-muscle Myosin IIA antibody [EPR22933-9] - BSA and Azide free (ab256477)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry kits

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human non-muscle Myosin IIA protein (Tagged) (ab226278)

Applications

Our Abpromise guarantee covers the use of ab238131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 226 kDa (predicted molecular weight: 226 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is not recommended for human.

IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.
    • Tissue specificity

      In the kidney, expressed in the glomeruli. Also expressed in leukocytes.
    • Involvement in disease

      Defects in MYH9 are the cause of May-Hegglin anomaly (MHA) [MIM:155100]. MHA is an autosomal dominant macrothrombocytopenia characterized by thrombocytopenia, giant platelets and leukokyte inclusions appearing as highly parallel paracrystalline bodies.
      Defects in MYH9 are the cause of Sebastian syndrome (SBS) [MIM:605249]. SBS is an autosomal dominant macrothrombocytopenia characterized by thrombocytopenia, giant platelets and leukocyte inclusions that are smaller and less organized than in May-Hegglin anomaly.
      Defects in MYH9 are the cause of Fechtner syndrome (FTNS) [MIM:153640]. FTNS is an autosomal dominant macrothrombocytopenia characterized by thrombocytopenia, giant platelets and leukocyte inclusions that are small and poorly organized. Additionally, FTNS is distinguished by Alport-like clinical features of sensorineural deafness, cataracts and nephritis.
      Defects in MYH9 are the cause of Alport syndrome with macrothrombocytopenia (APSM) [MIM:153650]. APSM is an autosomal dominant disorder characterized by the association of ocular lesions, sensorineural hearing loss and nephritis (Alport syndrome) with platelet defects.
      Defects in MYH9 are the cause of Epstein syndrome (EPS) [MIM:153650]. EPS is an autosomal dominant disorder characterized by the association of macrothrombocytopathy, sensorineural hearing loss and nephritis.
      Defects in MYH9 are the cause of deafness autosomal dominant type 17 (DFNA17) [MIM:603622]. DFNA17 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA17 is characterized by progressive hearing impairment and cochleosaccular degeneration.
      Defects in MYH9 are the cause of macrothrombocytopenia with progressive sensorineural deafness (MPSD) [MIM:600208]. MPSD is an autosomal dominant disorder characterized by the association of macrothrombocytopathy and progressive sensorineural hearing loss without renal dysfunction.
      Note=Subjects with mutations in the motor domain of MYH9 present with severe thrombocytopenia and develop nephritis and deafness before the age of 40 years, while those with mutations in the tail domain have a much lower risk of noncongenital complications and significantly higher platelet counts. The clinical course of patients with mutations in the four most frequently affected residues of MYH9 (responsible for 70% of MYH9-related cases) were evaluated. Mutations at residue 1933 do not induce kidney damage or cataracts and cause deafness only in the elderly, those in position 702 result in severe thrombocytopenia and produce nephritis and deafness at a juvenile age, while alterations at residue 1424 or 1841 result in intermediate clinical pictures.
      Note=Genetic variations in MYH9 are associated with non-diabetic end stage renal disease (ESRD).
    • Sequence similarities

      Contains 1 IQ domain.
      Contains 1 myosin head-like domain.
    • Domain

      The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    • Post-translational
      modifications

      ISGylated.
    • Target information above from: UniProt accession P35579 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4627 Human
      • Entrez Gene: 17886 Mouse
      • Entrez Gene: 25745 Rat
      • Omim: 160775 Human
      • SwissProt: P35579 Human
      • SwissProt: Q8VDD5 Mouse
      • SwissProt: Q62812 Rat
      • Unigene: 474751 Human
      • Unigene: 29677 Mouse
      • Unigene: 11385 Rat
      see all
    • Alternative names

      • BDPLT 6 antibody
      • BDPLT6 antibody
      • Cellular myosin heavy chain antibody
      • Cellular myosin heavy chain type A antibody
      • DFNA 17 antibody
      • DFNA17 antibody
      • EPSTS antibody
      • FTNS antibody
      • MGC104539 antibody
      • MHA antibody
      • MYH 2A antibody
      • MYH 9 antibody
      • MYH2A antibody
      • MYH9 antibody
      • MYH9_HUMAN antibody
      • MYHas8 antibody
      • MyHC 2A antibody
      • MyHC IIa antibody
      • MyHC2A antibody
      • MyHCIIa antibody
      • MYHSA 2 antibody
      • MYHSA2 antibody
      • Myosin 9 antibody
      • Myosin heavy chain 9 antibody
      • Myosin heavy chain 9 non muscle antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain non muscle IIa antibody
      • Myosin heavy chain nonmuscle IIa antibody
      • Myosin heavy polypeptide 2 antibody
      • Myosin heavy polypeptide 9 non muscle antibody
      • Myosin-9 antibody
      • Myosin9 antibody
      • NMHC II A antibody
      • NMMHC A antibody
      • NMMHC II a antibody
      • NMMHC II-a antibody
      • NMMHC IIA antibody
      • NMMHC-A antibody
      • NMMHC-IIA antibody
      • NMMHCA antibody
      • Non muscle myosin heavy chain A antibody
      • Non muscle myosin heavy chain antibody
      • Non muscle myosin heavy chain II A antibody
      • Non muscle myosin heavy polypeptide 9 antibody
      • non-muscle IIa antibody
      • Non-muscle myosin heavy chain A antibody
      • Non-muscle myosin heavy chain IIa antibody
      • Nonmuscle myosin heavy chain A antibody
      • Nonmuscle myosin heavy chain II A antibody
      • type A antibody
      see all

    Images

    • Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
      Western blot - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
      All lanes : Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131) at 1/1000 dilution

      Lane 1 : Human liver tissue lysate 20ug
      Lane 2 : Human lung lysate at 20 µg
      Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
      Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
      Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
      Lane 6 : Mouse lung tissue lysate at 20 µg
      Lane 7 : Rat spleen tissue lysate at 20 µg
      Lane 8 : Rat lung tissue lysate at 20 µg

      Secondary
      Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
      Lanes 4-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 226 kDa
      Observed band size: 226 kDa


      Exposure time: 26 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
      Immunoprecipitation - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

      non-muscle Myosin IIA was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug with ab238131 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238131. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug

      Lane 2: ab238131 IP in NIH/3T3 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238131 in NIH/3T3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling non-muscle Myosin IIA with ab238131 at 1/500 dilution (0.854 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver (PMID: 11943476, 22558294, 17668046). The section was incubated with ab238131 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)
      Anti-non-muscle Myosin IIA antibody [EPR22933-9] (ab238131)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab238131? Please let us know so that we can cite the reference in this datasheet.

    ab238131 has not yet been referenced specifically in any publications.

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