Western blot abreview for Anti-non-muscle Myosin IIA antibody [EPR8965]

Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - other (293T)
Specification
293T
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1*PBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL pico substrate
Bands
Specific: 240 kDa
Exposure
1 minute(s) and 0 second(s)

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Submitted Aug 15 2014

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