Key features and details
- Rabbit polyclonal to non-muscle Myosin IIB/MYH10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-non-muscle Myosin IIB/MYH10 antibody
See all non-muscle Myosin IIB/MYH10 primary antibodies
DescriptionRabbit polyclonal to non-muscle Myosin IIB/MYH10
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human cerebellum tissue; Human liver and tonsil lysates; HEK293 cells.
This product was previously labelled as non-muscle Myosin IIB
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 229 kDa.|
FunctionCellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Tissue specificityIsoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain.
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
modificationsPhosphorylated by ABL2.
- Information by UniProt
- Cellular myosin heavy chain antibody
- Cellular myosin heavy chain type B antibody
- Cellular myosin heavy chain type B type B antibody
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling non-muscle Myosin IIB/MYH10 with ab204358 at 1/1000 dilution.
All lanes : Anti-non-muscle Myosin IIB/MYH10 antibody (ab204358) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 229 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized HEK293 cells labeling non-muscle Myosin IIB/MYH10 with ab204358 at 4 µg/ml (green).
ab204358 has been referenced in 1 publication.
- Chen P et al. The expression and functional activities of smooth muscle myosin and non-muscle myosin isoforms in rat prostate. J Cell Mol Med 22:576-588 (2018). PubMed: 28990332