Overview

  • Product name
    Anti-Nonspecific Cytotoxic Cells antibody [5C.6]
  • Description
    Mouse monoclonal [5C.6] to Nonspecific Cytotoxic Cells
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, Flow Cyt, Inhibition Assay, IPmore details
  • Species reactivity
    Reacts with: Rat, Horse, Cow, Dog, Pig, Tilapia, Catfish
  • Immunogen

    Other Immunogen Type corresponding to Nonspecific Cytotoxic Cells. Purified NCC cells from catfish.

  • Positive control
    • This antibody as been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation.
  • General notes

    U.S. Patent Numbers: 5,028,424 and 5,229,494.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity
    Purified IgM
  • Primary antibody notes
    Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
  • Clonality
    Monoclonal
  • Clone number
    5C.6
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 10 µg/ml.

By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein.

Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

Inhibition Assay Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

References

This product has been referenced in:
  • Jaso-Friedmann L  et al. NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells. Mol Immunol 34:955-65 (1997). Read more (PubMed: 9464530) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. I received the following information from the lab: We don’t have the specific protocol used to test this antibody in flow cytometry. This product was developed outside our lab, and upon checking our files, I was not able to find the specific protocol used to test this product in this application. ab2778 is a mouse IgM. If you are using an anti-Mouse IgG that is fluroescently labeled you would not be able to detect the IgM and thus see no signal. We suggest using an IgM isotype control and an anti-Mouse IgM fluorescently labeled secondary antibody to obtain flow signal. Please also consider that this product should be stored at – 20 deg C. It is shipped with a regular “wet” ice pack but should be frozen upon receipt. Upon first opening the vial the researcher should aliquot it so that each subsequent aliquot undergoes just one more freeze/thaw cycle, as these can damage protein. In addition, I would suggest titration the antibody (i.e. increasing the concentration), but the anti-IgM secondary might be the reason for obtaining no results. I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

I have been informed by the suppliers for ab37131 and ab2778 that we do not supply the standard proteins unfortunately. For ab2778, anti-Nonspecific Cytotoxic Cell antibody, the lab team has informed me that this antibody was actually tested in ELISA by a customer. The only information we have available is that the researcher used a peptide capture ELISA to determine the active site for the receptor protein NCCRP-1. The lab has suggested that if one wants to quantify NCCRP-1, recombinant peptides would have to be used. Additionally, please keep in mind that this antibody is an IgM isotype. If you experience problems with this product please do contact us, as we have a guarantee on the quality of the antibody in Rat, Catfish, Cow, Dog, Horse, Pig, and Tilapia in ELISA. For ab37131, as I mentioned we don´t have a Rainbow trout CYP1A protein standards. However, I was able to find the specific protocol to be used for ELISA, which I have added to our datasheet and also copied below for your convenience. BUFFERS/REAGENTS 1. Coating buffer:50 mM carbonate-bicarbonate, pH 9.6. 2. PBS:Phosphate buffered saline, pH 7.3 3. Washing buffer: PBS, 0.05% Tween-20 4. Blocking/Dilution buffer: 1% BSA in PBS. 5. Substrate solution. I. ASSAY PROCEDURE Day 1: Coating with samples and positive control Frozen samples and positive control should be thawed on ice. 1. Dilute samples in Coating buffer: Please note: The optimum coating concentration must be determined for each assay, but these dilutions/concentrations can be generally recommended: Liver microsomes: 10 µg/ml Post-mitochondrial supernatants (PMS): 40 µg/ml Plasma samples: 1:1000 Purified proteins: 5-10 µg/ml 2. Dilute the positive control in coating buffer to reach desired concentration. 3. Add 100 µl coating buffer to each of two wells (duplicate analysis) or three wells (triplicate analysis). These wells will be used to determine the Non- Specific Background signal (NSB). 4. Add 100 µl diluted positive control to each of two or three wells. 5. For each sample, add 100 µl to each of two or three wells. 6. Incubate at 4 °C overnight Please note: Coating for 1 hour at room temperature or 37ºC is possible, but this may alter the sensitivity of the assay depending on the nature of the sample. Day 2: Blocking the wells 7. Wash the wells 3 times with 300 µl Washing buffer per well. 8. Add 200 µl of the Blocking/Dilution buffer to each well. Incubate at room temperature for 30-60 min. Incubation with Primary antibody 9. Empty the wells. 10. Dilute the Primary antibody in Blocking/Dilution buffer. Add 100 µl of the antibody solution to each well. Incubate at 37 °C for 1 hour. Please note: Incubation with the Primary antibody may be performed for 2 hours at 37 °C or at 4°C overnight. Depending on the Primary antibody, this may alter the sensitivity of the assay. Incubation with Secondary antibody 11. Wash the wells 3 times with 300 µl Washing buffer per well. 12. Dilute the Secondary antibody in Blocking/Dilution buffer for each plate run in the assay. Add 100 µl of the antibody solution to each well. Incubate at room temperature for 1 hour. Please note: Increasing the incubation time of the Secondary antibody to 2 hours or incubating at 37ºC may increase assay sensitivity. Development of the plate Please note: The Substrate solution should be prepared just before proceeding to the next step. 13. Wash 5 times with 300 µl Washing buffer per well. 14. Add 100 µl substrate solution to each well. Incubate at room temperature for 15 min Please note: If the reaction is weak, the incubation time may be extended up to 30 min. 15. Stop the reaction by adding 50 µl 2M H2SO4 to each well. 16. Read the absorbance at 492 nm. I hope this information will help you, good luck with your research.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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