Key features and details
- Rabbit polyclonal to NOP10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NOP10 antibody
See all NOP10 primary antibodies
DescriptionRabbit polyclonal to NOP10
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: HEK-293T, HeLa, Jurkat and Caco-2 whole cell lysates. IHC-P: Human breast cancer and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 8 kDa.|
FunctionRequired for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Involvement in diseaseDefects in NOP10 are a cause of dyskeratosis congenita autosomal recessive (ARDKC) [MIM:224230]. ARDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Sequence similaritiesBelongs to the NOP10 family.
Cellular localizationNucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
- Information by UniProt
- DKCB1 antibody
- H/ACA ribonucleoprotein complex subunit 3 antibody
- homolog of yeast Nop10p antibody
All lanes : Anti-NOP10 antibody (ab235339) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 8 kDa
Paraffin-embedded human breast cancer tissue stained for NOP10 with ab235339 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for NOP10 with ab235339 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235339 has not yet been referenced specifically in any publications.