Overview

  • Product name

    Anti-NOP10 antibody [EPR8856]
    See all NOP10 primary antibodies
  • Description

    Rabbit monoclonal [EPR8856] to NOP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NOP10 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human breast and papillary adenocarcinoma tissues; Jurkat, MOLT4, Raji and HeLa cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8856
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 7 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
    • Involvement in disease

      Defects in NOP10 are a cause of dyskeratosis congenita autosomal recessive (ARDKC) [MIM:224230]. ARDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
    • Sequence similarities

      Belongs to the NOP10 family.
    • Cellular localization

      Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKCB1 antibody
      • H/ACA ribonucleoprotein complex subunit 3 antibody
      • homolog of yeast Nop10p antibody
      • MGC70651 antibody
      • NOLA3 antibody
      • NOP10 antibody
      • NOP10 ribonucleoprotein homolog (yeast) antibody
      • NOP10_HUMAN antibody
      • NOP10P antibody
      • Nucleolar protein 10 antibody
      • Nucleolar protein family A member 3 antibody
      • nucleolar protein family A, member 3 (H/ACA small nucleolar RNPs) antibody
      • snoRNP protein NOP10 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue staining NOP10 using ab133726 at a 1/250 dilution.
    • All lanes : Anti-NOP10 antibody [EPR8856] (ab133726) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 7 kDa

    • Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma tissue staining NOP10 using ab133726 at a 1/250 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Nguyen THD  et al. Cryo-EM structure of substrate-bound human telomerase holoenzyme. Nature N/A:N/A (2018). Read more (PubMed: 29695869) »
    • Quan Wang J  et al. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem 33:1199-1211 (2018). Read more (PubMed: 30132373) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human embryonic stem cell, 293T, HeLa)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    50 µg
    Specification
    human embryonic stem cell, 293T, HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Jianlong Wang

    Verified customer

    Submitted Mar 19 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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