Overview

  • Product name

    Anti-NOP10 antibody [EPR8857]
    See all NOP10 primary antibodies
  • Description

    Rabbit monoclonal [EPR8857] to NOP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human NOP10 (UniProt: Q9NPE3).

  • Positive control

    • 293T, HeLa, Jurkat, HuT-78, and Caco 2 cell lysates. Human breast and kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.50 x 10 -12 M
    Learn more about KD
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8857
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 7 kDa.
ICC 1/250 - 1/500.

Target

  • Function

    Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
  • Involvement in disease

    Defects in NOP10 are a cause of dyskeratosis congenita autosomal recessive (ARDKC) [MIM:224230]. ARDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
  • Sequence similarities

    Belongs to the NOP10 family.
  • Cellular localization

    Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKCB1 antibody
    • H/ACA ribonucleoprotein complex subunit 3 antibody
    • homolog of yeast Nop10p antibody
    • MGC70651 antibody
    • NOLA3 antibody
    • NOP10 antibody
    • NOP10 ribonucleoprotein homolog (yeast) antibody
    • NOP10_HUMAN antibody
    • NOP10P antibody
    • Nucleolar protein 10 antibody
    • Nucleolar protein family A member 3 antibody
    • nucleolar protein family A, member 3 (H/ACA small nucleolar RNPs) antibody
    • snoRNP protein NOP10 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling NOP10 with ab134902 at 1/250 dilution.
  • All lanes : Anti-NOP10 antibody [EPR8857] (ab134902) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HuT-78 cell lysate
    Lane 5 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution

    Predicted band size: 7 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling NOP10 with ab134902 at 1/250 dilution.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

ab134902 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (human embryonic stem cell, 293T, HeLa)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
50 µg
Specification
human embryonic stem cell, 293T, HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Jianlong Wang

Verified customer

Submitted Mar 09 2018

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12 % GEL, SDS)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Apr 18 2014

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