Recombinant
RabMAb

Recombinant Anti-NOP58 antibody [EPR10721] (ab155969)

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Overview

  • Product name

    Anti-NOP58 antibody [EPR10721]
    See all NOP58 primary antibodies

  • Description

    Rabbit monoclonal [EPR10721] to NOP58

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to residues in Human NOP58 (Uniprot: Q9Y2X3).

  • Positive control

    • HeLa, K562, MCF7 and NCI-H460 cell lysates; Human hepatocellular carcinoma tissue; MCF7 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    Images

    • Western blot - Anti-NOP58 antibody [EPR10721] (ab155969)
      Western blot - Anti-NOP58 antibody [EPR10721] (ab155969)
      All lanes : Anti-NOP58 antibody [EPR10721] (ab155969) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : NCI-H450 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue, labeling NOP58 with ab155969 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)
      Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)
      Immunofluorescence analysis of MCF7 cells, labeling NOP58 with ab155969 at 1/250 dilution.

    References

    ab155969 has not yet been referenced specifically in any publications.

    Publishing research using ab155969? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-NOP58 antibody [EPR10721]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HES2 human embryonic stem cell, 293T, HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    HES2 human embryonic stem cell, 293T, HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Jianlong Wang

    Verified customer

    Submitted Dec 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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