Product nameAnti-NOP58 antibody [EPR10721]
See all NOP58 primary antibodies
DescriptionRabbit monoclonal [EPR10721] to NOP58
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human NOP58 (Uniprot: Q9Y2X3).
- HeLa, K562, MCF7 and NCI-H460 cell lysates; Human hepatocellular carcinoma tissue; MCF7 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab155969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionRequired for 60S ribosomal subunit biogenesis.
Sequence similaritiesBelongs to the NOP5/NOP56 family.
Contains 1 Nop domain.
modificationsSumoylation is essential for high-affinity binding to snoRNAs.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- HSPC120 antibody
- NOL5 antibody
- NOP5 antibody
All lanes : Anti-NOP58 antibody [EPR10721] (ab155969) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : NCI-H450 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue, labeling NOP58 with ab155969 at 1/50 dilution.
Immunofluorescence analysis of MCF7 cells, labeling NOP58 with ab155969 at 1/250 dilution.
ab155969 has not yet been referenced specifically in any publications.