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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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RecombinantRabMAb

Recombinant Anti-NOP58 antibody [EPR10721] (ab155969)

  • Datasheet
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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-NOP58 antibody [EPR10721] (ab155969)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)
  • Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)
  • Anti-NOP58 antibody [EPR10721] (ab155969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10721] to NOP58
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Overview

  • Product name

    Anti-NOP58 antibody [EPR10721]
    See all NOP58 primary antibodies
  • Description

    Rabbit monoclonal [EPR10721] to NOP58
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human NOP58 (Uniprot: Q9Y2X3).

  • Positive control

    • HeLa, K562, MCF7 and NCI-H460 cell lysates; Human hepatocellular carcinoma tissue; MCF7 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10721
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Alternative Versions

    • Anti-NOP58 antibody [EPR10721] - BSA and Azide free (ab249222)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K562 whole cell lysate (ab7911)

Applications

Our Abpromise guarantee covers the use of ab155969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Required for 60S ribosomal subunit biogenesis.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the NOP5/NOP56 family.
      Contains 1 Nop domain.
    • Post-translational
      modifications

      Sumoylation is essential for high-affinity binding to snoRNAs.
    • Cellular localization

      Nucleus > nucleolus.
    • Target information above from: UniProt accession Q9Y2X3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51602 Human
      • Entrez Gene: 55989 Mouse
      • Entrez Gene: 60373 Rat
      • SwissProt: Q9Y2X3 Human
      • SwissProt: Q6DFW4 Mouse
      • SwissProt: Q9QZ86 Rat
      • Unigene: 471104 Human
      • Unigene: 220367 Mouse
      • Unigene: 43930 Rat
      see all
    • Alternative names

      • HSPC120 antibody
      • NOL5 antibody
      • NOP5 antibody
      • NOP5/NOP58 antibody
      • NOP58 antibody
      • NOP58 ribonucleoprotein homolog (yeast) antibody
      • NOP58 ribonucleoprotein homolog antibody
      • NOP58_HUMAN antibody
      • Nucleolar protein 5 antibody
      • Nucleolar protein 58 antibody
      • Nucleolar protein NOP5/NOP58 antibody
      see all

    Images

    • Western blot - Anti-NOP58 antibody [EPR10721] (ab155969)
      Western blot - Anti-NOP58 antibody [EPR10721] (ab155969)
      All lanes : Anti-NOP58 antibody [EPR10721] (ab155969) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : NCI-H450 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOP58 antibody [EPR10721] (ab155969)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue, labeling NOP58 with ab155969 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)
      Immunocytochemistry/ Immunofluorescence - Anti-NOP58 antibody [EPR10721] (ab155969)
      Immunofluorescence analysis of MCF7 cells, labeling NOP58 with ab155969 at 1/250 dilution.
    • Anti-NOP58 antibody [EPR10721] (ab155969)
      Anti-NOP58 antibody [EPR10721] (ab155969)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab155969? Please let us know so that we can cite the reference in this datasheet.

    ab155969 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-NOP58 antibody [EPR10721]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (hek293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    25 µg
    Specification
    hek293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Robert Kupp

    Verified customer

    Submitted Oct 20 2020

    Western blot abreview for Anti-NOP58 antibody [EPR10721]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HES2 human embryonic stem cell, 293T, HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    HES2 human embryonic stem cell, 293T, HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Jianlong Wang

    Verified customer

    Submitted Dec 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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