Product nameAnti-Noradrenaline antibody
DescriptionRabbit polyclonal to Noradrenaline
Specificityab8887 targets conjugated noradrenaline only it does not recognize free noradrenaline.
Tested applicationsSuitable for: ICC, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Noradrenaline conjugated to Bovine Serum Albumin (BSA).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: ddH20, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab8887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution (PMID 18457899).
IHC-Fr: Use at an assay dependent concentration.
The recommended dilution can be between 1:2,000-1:5,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceNoradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
- Arterenol antibody
- NA antibody
- Norepinephrine antibody
ab8887 staining Noradrenaline (red) in rat smooth muscle cells from the aorta.
Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.
This product has been referenced in:
- Song W et al. Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue. PLoS Biol 17:e2006571 (2019). Read more (PubMed: 30653498) »
- Renz BW et al. ß2 Adrenergic-Neurotrophin Feedforward Loop Promotes Pancreatic Cancer. Cancer Cell 33:75-90.e7 (2018). Read more (PubMed: 29249692) »