Overview

  • Product name
    Anti-Noradrenaline antibody
  • Description
    Rabbit polyclonal to Noradrenaline
  • Host species
    Rabbit
  • Specificity
    ab8887 targets conjugated noradrenaline only it does not recognize free noradrenaline.
  • Tested applications
    Suitable for: ICC, IHC-Fr, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Noradrenaline conjugated to Bovine Serum Albumin (BSA).

Properties

Applications

Our Abpromise guarantee covers the use of ab8887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
IHC-Fr
IHC-P
  • Application notes
    ELISA: Use at an assay dependent concentration.
    ICC: Use at an assay dependent concentration.
    IHC-P: Use at an assay dependent dilution (PMID 18457899).
    IHC-Fr: Use at an assay dependent concentration.

    The recommended dilution can be between 1:2,000-1:5,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Noradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
    • Cellular localization
      Secreted
    • Alternative names
      • Arterenol antibody
      • NA antibody
      • Norepinephrine antibody

    Images

    • ab8887 staining Noradrenaline (red) in rat smooth muscle cells from the aorta.

      Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.

    References

    This product has been referenced in:
    • Yadirgi G  et al. Immuno-detection of cleaved SNAP-25 from differentiated mouse embryonic stem cells provides a sensitive assay for determination of botulinum A toxin and antitoxin potency. J Immunol Methods 451:90-99 (2017). ICC/IF . Read more (PubMed: 28943257) »
    • Kondo Y  et al. Expression and Role of the BDNF Receptor-TrkB in Rat Adrenal Gland under Acute Immobilization Stress. Acta Histochem Cytochem 43:139-47 (2010). ICC/IF . Read more (PubMed: 21245980) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer

    Thank you for your enquiry and interest in our products.

    The storage buffer of this antibody only contains Glycerol which is nonhazardous, there is no MSDS for ab8887. A general SDS document can be downloaded from the on-line datasheet.

    I would like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Answer

    Thank you very much for your reply.

    For ab6454, it looks like the antibody was tested with glutaraldehyde-perfused tissue sections. There is a link to "Immunohisto and cytochemistry" protocol at the very bottom of the datasheet-

    https://www.abcam.com/noradrenaline-antibody-ab6454.html

    Looking through the references of ab8887, one shows fixation with PFA-glutaraldehyde-

    http://endo.endojournals.org/content/145/4/1750.long

    The other referenced protocols using ab8887 are not as clear in regards to the fixative used in IHC. I would suggest using a PFA-glutaraldehyde fixation based on these protocols.

    I hope that ths information will be useful, but if you have further questions or need anything else, please let me know and I'll be happy to help. Have a nice day!

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    Answer

    Thank you very much for contacting us with your enquiry.

    I apologize for the confusion regarding these products. Both ab8887 and ab6454 are antibodies that target conjugated noradrenaline, instead of free noradrenaline. As a small molecule, NA does not induce an immune response, so the immunogen of these antibodies was NA conjugated to BSA via a glutaraldehyde linker. Neither of them is conjugated to a fluorophore or other detection molecule.

    The main difference between the two antibodies is that ab8887 is a purified IgG fraction, and ab6454 is unpurified whole antiserum. Both of them have been tested with frozen tissue (IHC-Fr). Will you be perfusing the tissue with PFA, or post-fixing?

    For fluorescent staining, you will need to use a fluorescent-conjugated secondary antibody, such as ab6717-

    https://www.abcam.com/rabbit-igg-secondary-antibody-h-l-ab6717.html

    I hope that this information will be useful, but please let me know if you have further questions or need anything else, and I'll be happy to help.

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    Answer

    Thank you for contacting Abcam.


    Attached please find the MSDS you requested.


    Please do not hesitate to contact me if you have any additional questions.

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    Question
    Answer

    Thank you for contacting Abcam regarding ab8887.

    I have confirmed with the laboratory that unfortunately, there is no concentration information available for this antibody.

    I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Answer

    Thank you for your enquiry. The FAQ you noticed is from Thursday 25-November-2004 at which time the antibody had not been tested in IHC-P. We since have data from a publication that came to our attention in Sept 2008. PMID 18457899. This publication has used the antibody successfully in IHC-P. We therefore added IHC-P as a tested application and I can confirm we provide our Abpromise guarantee for this antibody in IHC-P. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Answer

    Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested this ourselves. We invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. I hope this information helps and please let me know how the ELISAs work.

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    Question

    Thank you for your questions. I would like to answer them one by one. > 1. Please describe the problem (high background, no staining etc). answer: no staining, no positive results. > 2. On what material are you testing the antibody in IHC? answer: human and rat bladder Tissue > > 3. How did you fix the samples? answer: Paraformaldehyde > > 4. Did you apply antigen retrieval step? answer: I do not think this procedure is needed, since I used fresh cryostat sections. > > 5. How did you block the unspecific binding sites? answer: using normal horse serum > > 6. Primary antibody Specification (in which species was it raised against)? answer: human and rat At what dilution(s) have you tested this antibody? answer: from 1:200, 1:400, 1:1000, 1:2000, to 1:4000. Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? answer: incubation at 4c for 16-18 hours, or room tempereture for 16 to 18 hours. wash steps: each time 5 minutes for 3 times. > > 7. Secondary antibody What secondary antibody are you using? answer: "Histofine simple stain Max(R)" from Novocastra company or "Alexa Fluore 594 donkey anti-rabbit IgG" from Molecular Probe. Specification (in which species was it raised against)? answer: bladder including neck and dome. What detection method are you using? answer: both DAB staining and immunoflorencent methods. > 8. Background staining Please provide an image of your staining answer: the image is attached in the attachment. > > 9. Which detection system did you use? answer: DAB staining and immunoflorencent > 10. Did you apply positive and negative controls along with the samples? Please specify. answer: Yes, for positive control, I used other antibodys such as TH, for staining of noradenergic nerve fibers in the same series sections, and got good positive results. For negative controls, I used the normal rabbit serum to replace the primary antibody. > > 11. Optimization attempts How many times have you tried the IHC? answer: for more than 5 times. Do you obtain the same results every time? answer: yes. What steps have you altered? answer: different dilution(s) of primary antibody, and different incubation times, and different decondary detecting systems. Thank you for your comprehensive questions. I hope you will be satisfied with my answers. I am looking forward to seeing your answer again.

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    Answer

    Thank you for your patience and forwarding your detailed protocol on-line. We are very sorry to hear that you are having some problem with this antibody. Looking through your email, I can understand that you have fixed the tissue sections in paraformaldehyde. As a general rule, formalin fixed tissue requires an antigen retrieval step before immunohistochemical staining can proceed. This is due to the formation of methylene bridges during fixation, which cross link proteins and therefore mask antigenic sites. This particular antibody has not been tested for IHC on formalin-fixed sections only on frozen section. We would therefore suggest trying different fixatives: either ice-cold acetone (for 5 or 10 min), or methanol or in ethanol. There are two specific publications listed on the datasheet, please take a look at them; you may find some useful information in them. We need to emphasize that each antibody has different specificity and characteristics so even if another antibody (like TH you mentioned in your message) works nicely in the same series sections, it does not necessary mean that this antibody is suitable for IHC on formalin-fixed sections. We hope this will help you. Should you still have problem with this product, please do not hesitate to contact us again.

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