Key features and details
- Rabbit polyclonal to Noradrenaline transporter
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Noradrenaline transporter antibody
See all Noradrenaline transporter primary antibodies
DescriptionRabbit polyclonal to Noradrenaline transporter
SpecificityAb41559 does not cross react with dopamine and serotonin transporters.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
The noradrenaline transporter antibodies were generated against unique N-terminal peptides that are unique to the noradrenaline transporter protein. The antibodies are raised utilizing linear, multiple antigenic peptides or cyclic peptide methodology.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris glycine, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab41559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionAmine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.
Involvement in diseaseOrthostatic intolerance
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.
- Information by UniProt
- NAT1 antibody
- NET antibody
- NET1 antibody
Anti-Noradrenaline transporter antibody (ab41559) at 1/250 dilution + rat brain cortical membrane cell lysate at 70 µg
Predicted band size: 70 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.
Western Blot with rat brain cortical membranes
ICC/IF image of ab41559 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41559, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab41559 has been referenced in 1 publication.
- Jovanovic P et al. Oxytocin modulates the expression of norepinephrine transporter, ß3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. Peptides N/A:N/A (2018). PubMed: 29969648