Overview

  • Product name
    Normal Bovine Calf Serum (sterile)
    See all Bovine Serum reagents
  • Host species
    Cow
  • Tested applications
    Suitable for: Blocking, IHC-P, IHC-Frmore details
  • General notes

    Total Protein Concentration : normal. Strain: Mixed breed and sex

    Raised in: Cow

    Purity: Whole antiserum

    Sterilization: This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Properties

Applications

Our Abpromise guarantee covers the use of ab7479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References

ab7479 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Both of these sera were producted using starting materials of North American origin in USDA inspected facilities.

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Answer

Thank you for your enquiry. The product ab7479 is not bovine serum albumin but bovine serum therefore it contains more proteins that just albumin. This product is typically used to reduce non-specific background staining which may be caused by a bovine secondary antibody during immunohistochemistry staining procedures. It may however work in western blotting. We typically recommend a 10% dilution (10ml serum plus 90ml PBST) as blocking agent for immunohistochemistry so I would recommend trying 5% and 10% (in TBST rather than PBST) and a one hour incubation. Please let me know if you need further help,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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