Normal Donkey Serum (ab7475)
Overview
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Product name
Normal Donkey Serum
See all Donkey Serum reagents -
Host species
Donkey -
Tested applications
Suitable for: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
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General notes
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.
Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.
Serum can be used directly for blocking, or as a constituent of a blocking buffer.
Strain: Mixed breed and sex.
Raised in: Donkey
Purity: Whole antiserum
Properties
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Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Research areas
Associated products
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Related Products
- Normal Donkey serum (sterile) (ab138579)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
- Donkey Anti-Goat IgG H&L (HRP) (ab97110)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC (PFA fixed) |
Use at an assay dependent dilution.
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| Blocking |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.
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| Notes |
|---|
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IHC (PFA fixed)
Use at an assay dependent dilution. |
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Blocking
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution. |
Images
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Functional Studies - Normal Donkey Serum (ab7475)Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285
The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.
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Functional Studies - Normal Donkey Serum (ab7475)Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (128)
ab7475 has been referenced in 128 publications.
- Alvarez KLF et al. Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer's disease. STAR Protoc 3:101149 (2022). PubMed: 35141568
- Privat-Maldonado A et al. Cold Atmospheric Plasma Does Not Affect Stellate Cells Phenotype in Pancreatic Cancer Tissue in Ovo. Int J Mol Sci 23:N/A (2022). PubMed: 35216069
- Zywitza V et al. Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction. Sci Rep 12:3100 (2022). PubMed: 35260583
- Nemeth DP et al. Dynamic Interleukin-1 Receptor Type 1 Signaling Mediates Microglia-Vasculature Interactions Following Repeated Systemic LPS. J Inflamm Res 15:1575-1590 (2022). PubMed: 35282272
- Abdel-Razek O et al. Role of Surfactant Protein D in Experimental Otitis Media. J Innate Immun 13:197-210 (2021). PubMed: 33556949
