Overview

  • Product name

    Normal Rabbit Serum (Sterile)
    See all Rabbit serum reagents
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Blocking, IHC-P, IHC-Frmore details
  • General notes

    Sterile rabbit serum ab7487 for blocking in antibody-based applications or use as an antibody diluent.

    Origin: North America.

    Total Protein Concentration : 78 mg/ml (by Refractometry). Strain: New Zealand White. Sex: Mixed sex

    Raised in: Rabbit

    Purity: Whole antiserum

Properties

Applications

Our Abpromise guarantee covers the use of ab7487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References

This product has been referenced in:

  • Xu Y  et al. Differentiation of CD45-/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43:1128-1138 (2019). Read more (PubMed: 30628669) »
  • Tie D  et al. Bullous Pemphigoid IgG Induces Cell Dysfunction and Enhances the Motility of Epidermal Keratinocytes via Rac1/Proteasome Activation. Front Immunol 10:200 (2019). Read more (PubMed: 30809225) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

sterile serum for blocking

Excellent Excellent 5/5 (Ease of Use)
Abreviews
good for blocking variability of immunofluorescent staining.
5-10% dilution for 1 hour in RT is recommended.

Abcam user community

Verified customer

Submitted Nov 11 2019

very effective blocking

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I use it to effectively block in IHC-P staining, usually 10% serum in TBST is sufficient.

Abcam user community

Verified customer

Submitted Jul 04 2019

Answer

Thank you for contacting us.

Generally the serum is OK for 1 year at 4C and for 10 years when stored at -20C.

Please check the waste disposal policy of your organization. We do not include this information in MSDS, this should be taken care of by the laboratory where these substances are being used.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

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https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

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https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

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https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Answer



Veuillez trouver ci-joints les certificats d'analyse pour les produits :

Rabbit serum ref : ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1

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Question
Answer

Thank you for contacting us.

I have attached the CoC for AB7487 as you have requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your inquiry.
Yes, I am happy to confirm that rabbit serum ab7487 can be used for blocking in IP.
I hope this information is helpful. Please let me know if you would need some additional or more specific information.
I wish you a good day!

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Answer

Thank you for contacting Abcam.
Those values are for whole protein value which we supply as a customer service. The total protein value is not considered when making blocking solutions as the percentage used is of the whole serum. Therefore to create a 100 ml or a 10% IHC block you would use 10 ml of serum in 90 ml of diluent.
I hope this information is helpful. Please let me know if you have any questions, concerns or there is any other way that I might help you.

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Answer

Thank you for your inquiry.
According to the standard protocol we have for free floating sections, we recommend to incubate the sections with 0.1M PB/0.3% triton/1% blocking serum for at least 1 h.
The whole protocol can be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=13633

As always certain steps including the amount of blocking agent might have to be optimized according to circumstances like sample, results, etc.
I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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Answer

Thank you for your inquiry.
I assume you would like to use this serum for IHC in the blocking step?
In this case the serum does not have to be heat treated. The heat treatment is done to inactivate complement in FCS meant for cell culture for example.
I recommend to just aliquot and freeze the serum.
I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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1-10 of 14 Abreviews or Q&A

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