Overview

  • Product name

    Anti-Norovirus GII.4 antibody [17E11]
    See all Norovirus GII.4 primary antibodies
  • Description

    Mouse monoclonal [17E11] to Norovirus GII.4
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    VLP GII.4 Minerva 2006.

  • General notes

    pI: 4.6-7.0

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    17E11
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Noroviruses (NoV) are a genetically diverse group of single stranded RNA, nonenveloped viruses belonging to the Caliciviridae family. Noroviruses can genetically be classified into 5 different genogroups (GI, GII, GIII, GIV, and GV) which can be further divided into different genetic clusters or genotypes. For example genogroup II, the most prevalent human genogroup, presently contains 19 genotypes. Genogroups I, II and IV infect humans, whereas genogroup III infects bovine species and genogroup V has recently been isolated in mice. Noroviruses commonly isolated in cases of acute gastroenteritis belong to two genogroups: genogroup I (GI) includes Norwalk virus, Desert Shield virus and Southampton virus and II (GII) which includes Bristol virus, Lordsdale virus, Toronto virus, Mexico virus, Hawaii virus and Snow Mountain virus. Noroviruses contain a positive-sense RNA genome of approximately 7.5 knt, encoding a major structural protein (VP1) of about 58~60 kDa and a minor capsid protein (VP2). The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy and are between 27-38 nm in size.
  • Alternative names

    • NoV antibody

References

ab125039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
6 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Pichia pastoris Recombinant protein (Whole cells)
Specification
Whole cells
Treatment
1% Methanol for 24hrs
Blocking step
Gelatin-Net as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2014

Answer


Both antibodies were raised against VLP GII.4 Minerva 2006. Both antibodies differ in the clone number.

Also, ab125151 is specific to GII.4 VLP variants from 1987-2006, while ab125039 is specific to GII.4 VLP variants from 2002-2010.

Read More

Answer

Thank you for contacting us.

The antibodies have only been tested in ELISA. We do not have any data for western blotting.

Several of these are monoclonal antibodies that are raised against various strains of Norovirus, and the epitopes that they recognize are unknown, so we are not able to predict their potential for reactivity with the sequence you provided. These antibodies are ab64042, ab125039, and ab125151.

Two other antibodies, ab80022 and ab80024, are raised against peptides for which we do not have the amino acid sequences. I am sending your capsid protein VP1 human Norovirus GII-4 sequence to the laboratories that produced these clones to obtain alignments of the immunogen sequences with the VP1 sequence. I will send you the percentage identities when I receive them.

The one antibody whose immunogen sequence we have is ab92976. The sequence is

LTGQQNIIDPWIMNNFV.

Alignment with your sequence shows 76% identity:

+ GQQN+IDPWI NNFV

So, there is a possibility the antibody will cross-react with the Norovirus GII-4 capsid VP1.

I will send the information I receive for ab80022 and ab80024. If you have any questions, please contact me.

Read More

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