Overview

  • Product name

  • Description

    Goat polyclonal to NOS-1
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise the longer isoform 1 only, not isoform 2.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NOS-1 aa 281-292.
    Sequence:

    SARDGPPGKKLR

  • Positive control

    • WB: Human testis tissue lysate.
  • General notes

    Previously labelled as Nanos Homologue 1 (NANOS1). 

Properties

Applications

Our Abpromise guarantee covers the use of ab36163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
IHC-P Use a concentration of 2.5 µg/ml. PubMed: 18223680

Target

  • Function

    May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
  • Tissue specificity

    Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
  • Sequence similarities

    Belongs to the nanos family.
    Contains 1 nanos-type zinc finger.
  • Developmental stage

    Fetal ovary and fetal testis (at protein level).
  • Domain

    The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
    The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
  • Cellular localization

    Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC Rep1a antibody
    • EC_Rep1a antibody
    • NANO1_HUMAN antibody
    • NANOS 1 antibody
    • Nanos homolog 1 (Drosophila) antibody
    • Nanos homolog 1 antibody
    • Nanos Homologue 1 (NANOS1) antibody
    • NANOS1 antibody
    • Nos 1 antibody
    • NOS-1 antibody
    • Nos1 antibody
    • OTTHUMP00000046853 antibody
    • Rep 1a antibody
    • Rep1a antibody
    see all

Images

  • Anti-NOS-1 antibody (ab36163) at 0.3 µg/ml + Human testis tissue lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

This product has been referenced in:

  • Bonnomet A  et al. The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. Oncogene 27:3692-9 (2008). IHC-P ; Human . Read more (PubMed: 18223680) »
See 1 Publication for this product

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