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Rabbit polyclonal to NOSIP
Predicted to work with:
Mouse, Rat, Cow
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 17-270 of Human NOSIP (AAH09299).
293T, A431, Raji whole cell lysate; U87 xenograft
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
NOSIP is a novel type of modulator that promotes translocation of eNOS from the plasma membrane to intracellular sites, thereby uncoupling eNOS from plasma membrane caveolae and inhibiting NO synthesis.
Cytoplasm. Nucleus. Note: Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle.
CGI 25 antibody
CGI25 protein antibody
Western blot - Anti-NOSIP antibody (ab102087)
All lanes : Anti-NOSIP antibody (ab102087) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 33 kDa 12% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOSIP antibody (ab102087)
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ab102087 at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"